ArticleCurrent genetics1992
Isolation and characterization of the Aspergillus niger pyruvate kinase gene.
Article in Current genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
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- The Sugar Metabolic Model ofJournal of fungi (Basel, Switzerland) · 2022Article
- Metabolic engineering of an industrial3 Biotech · 2020Article
- The gold-standard genome ofStudies in mycology · 2018Article
- Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger.Microbial cell factories · 2014Article
- Heterologous expression of Gaeumannomyces graminis lipoxygenase in Aspergillus nidulans.AMB Express · 2014Article
- Biotechnological production of citric acid.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Differential expression of citA gene encoding the mitochondrial citrate synthase of Aspergillus nidulans in response to developmental status and carbon sources.Journal of microbiology (Seoul, Korea) · 2010Article
- Identification of amino acid residues critical for catalysis and stability in Aspergillus niger family 1 pectin lyase A.The Biochemical journal · 2003Article
- Structure and function of pectic enzymes: virulence factors of plant pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2000Review
- Two cellobiohydrolase-encoding genes from Aspergillus niger require D-xylose and the xylanolytic transcriptional activator XlnR for their expression.Applied and environmental microbiology · 1999Article
- Expression of an Erwinia pectate lyase in three species of Aspergillus.Current genetics · 1996Article
- Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger.Current genetics · 1994Article
- Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum.Applied and environmental microbiology · 1994Article
- Induction of glucose oxidase, catalase, and lactonase in Aspergillus niger.Current genetics · 1993Article
- Cloning of the Aspergillus niger gene encoding alpha-L-arabinofuranosidase A.Applied microbiology and biotechnology · 1993Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Aspergillus niger gene encoding pyruvate kinase was cloned by heterologous hybridization using a fragment from the corresponding yeast gene as a probe. The primary structure of the gene, including 5' and 3' flanking sequences, was determined. The structural part of the A. niger pkiA gene is 2054 bp long and is interrupted by seven putative introns. Splicing of the intron sequences results in an open reading frame of 1578 bp, encoding a protein of 526 amino-acid residues and a molecular weight of 58,130 Da. Extensive homology is found with pyruvate kinase from A. nidulans; only 33 amino acids are different between both proteins. Transformation experiments using the pyrA gene as a selection marker and the subcloned pkiA gene as a co-transforming marker led to increased levels of pyruvate kinase. Analysis of the transformants showed that in none of the transformants integration had occurred at the pkiA locus. Predominantly co-integration of the pyrA- and the pkiA-containing plasmids was found in the cases examined.
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