ArticleVirology journal2005
Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene.
Article in Virology journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters.Cytotechnology · 2016Article
- The Pacific White Shrimp β-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus.Marine biotechnology (New York, N.Y.) · 2016Article
- Transcript Analysis of White spot syndrome virus Latency and Phagocytosis Activating Protein Genes in Infected Shrimp (Penaeus monodon).Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine.BMC microbiology · 2008Article
- Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1.Journal of virology · 2008Article
- White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1.Journal of virology · 2007Article
- In silico identification of putative promoter motifs of White Spot Syndrome Virus.BMC bioinformatics · 2006Article
- White spot syndrome virus: an overview on an emergent concern.Veterinary researchArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhite spot syndrome virus (WSSV) is an important pathogen of the penaeid shrimp with high mortalities. In previous reports, Orf427 of WSSV is characterized as one of the three major latency-associated genes of WSSV. Here, we were interested to analyze the promoter of orf427 and its expression during viral pathogenesis.
resultsin situ hybridization revealed that orf427 was transcribed in all the infected tissues during viral lytic infection and the translational product can be detected from the infected shrimp. A time-course RT-PCR analysis indicated that transcriptional products of orf427 could only be detected after 6 h post virus inoculation. Furthermore, a baculovirus-mediated promoter analysis indicated that the promoter of orf427 failed to express the EGFP reporter gene in both insect SF9 cells and primary shrimp cells.
conclusionOur data suggested that latency-related orf427 might not play an important role in activating virus replication from latent phase due to its late transcription during the lytic infection.
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Registered trials
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