ArticleInfection and immunity2005
Cable pili and the 22-kilodalton adhesin are required for Burkholderia cenocepacia binding to and transmigration across the squamous epithelium.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Quorum sensing and DNA methylation play active roles in clinicalJournal of bacteriology · 2025Article
- In-Silico Analysis Highlights the Existence in Members ofMicroorganisms · 2023Article
- Genomic features, antimicrobial susceptibility, and epidemiological insights intoFrontiers in cellular and infection microbiology · 2023Article
- Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells.Scientific reports · 2021Article
- Article
- Burkholderia cenocepacia-host cell contact controls the transcription activity of the trimeric autotransporter adhesin BCAM2418 gene.MicrobiologyOpen · 2020Article
- Genomic analyses of Burkholderia cenocepacia reveal multiple species with differential host-adaptation to plants and humans.BMC genomics · 2019Article
- Investigating the Mechanism of Action of Diketopiperazines Inhibitors of theFrontiers in pharmacology · 2018Article
- Prospects for subunit vaccines: Technology advances resulting in efficacious antigens requires matching advances in early clinical trial investment.Human vaccines & immunotherapeutics · 2016Article
- Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis.MicrobiologyOpen · 2016Article
- Mechanisms of Disease: Host-Pathogen Interactions between Burkholderia Species and Lung Epithelial Cells.Frontiers in cellular and infection microbiology · 2015Review
- Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance.Frontiers in microbiology · 2015Review
- Article
- Identification of functions linking quorum sensing with biofilm formation in Burkholderia cenocepacia H111.MicrobiologyOpen · 2012Article
- Molecular mechanisms of chlorhexidine tolerance in Burkholderia cenocepacia biofilms.Antimicrobial agents and chemotherapy · 2011Article
- Cable pili and the associated 22 kDa adhesin contribute to Burkholderia cenocepacia persistence in vivo.PloS one · 2011Article
- Burkholderia cepacia Complex: Emerging Multihost Pathogens Equipped with a Wide Range of Virulence Factors and Determinants.International journal of microbiology · 2011Article
- Host evasion by Burkholderia cenocepacia.Frontiers in cellular and infection microbiology · 2011Review
- Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Burkholderia cenocepacia strains expressing both cable (Cbl) pili and the 22-kDa adhesin bind to cytokeratin 13 (CK13) strongly and invade squamous epithelium efficiently. It has not been established, however, whether the gene encoding the adhesin is located in the cbl operon or what specific contribution the adhesin and Cbl pili lend to binding and transmigration or invasion capacity of B. cenocepacia. By immunoscreening an expression library of B. cenocepacia isolate BC7, we identified a large gene (adhA) that encodes the 22-kDa adhesin. Isogenic mutants lacking expression of either Cbl pili (cblA or cblS mutants) or the adhesin (adhA mutant) were constructed to assess the individual role of Cbl pili and the adhesin in mediating B. cenocepacia binding to and transmigration across squamous epithelium. Relative to the parent strain, mutants of Cbl pili showed reduced binding (50%) to isolated CK13, while the adhesin mutant showed almost no binding (0 to 8%). Mutants lacking either cable pili or the adhesin were compromised in their ability to bind to and transmigrate across the squamous epithelium compared to the wild-type strain, although this deficiency was most pronounced in the adhA mutant. These results indicate that both Cbl pili and the 22-kDa adhesin are necessary for the optimal binding to CK13 and transmigration properties of B. cenocepacia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.