ArticlePlant physiology2005
Dedifferentiation of tobacco cells is associated with ribosomal RNA gene hypomethylation, increased transcription, and chromatin alterations.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Spatiotemporal transcriptomic atlas of rhizome formation in Oryza longistaminata.Plant biotechnology journal · 2024Article
- Enigmatic role of auxin response factors in plant growth and stress tolerance.Frontiers in plant science · 2024Review
- Personal Perspectives on Plant Ribosomal RNA Genes Research: From Precursor-rRNA to Molecular Evolution.Frontiers in plant science · 2021Review
- Global Transcriptome and Coexpression Network Analyses Reveal New Insights Into Somatic Embryogenesis in Hybrid Sweetgum (Frontiers in plant science · 2021Article
- Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology?Frontiers in plant science · 2019Review
- Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.Plant cell reports · 2016Article
- DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.).Journal of biological research (Thessalonike, Greece) · 2016Article
- The origin of exon 3 skipping of paternal GLOBOSA pre-mRNA in some Nicotiana tabacum lines correlates with a point mutation of the very last nucleotide of the exon.Molecular genetics and genomics : MGG · 2016Article
- Seed response to strigolactone is controlled by abscisic acid-independent DNA methylation in the obligate root parasitic plant, Phelipanche ramosa L. Pomel.Journal of experimental botany · 2015Article
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- Plant callus: mechanisms of induction and repression.The Plant cell · 2013Review
- Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture.Epigenetics · 2013Article
- An evolutionary view of plant tissue culture: somaclonal variation and selection.Plant cell reports · 2012Review
- ABA-mediated inhibition of seed germination is associated with ribosomal DNA chromatin condensation, decreased transcription, and ribosomal RNA gene hypoacetylation.Plant molecular biology · 2012Article
- Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications.Plant cell reports · 2012Review
- TCUP: A Novel hAT Transposon Active in Maize Tissue Culture.Frontiers in plant science · 2012Article
- DNA methylation and histone modifications regulate de novo shoot regeneration in Arabidopsis by modulating WUSCHEL expression and auxin signaling.PLoS genetics · 2011Article
- Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression.BMC plant biology · 2009Article
- Faithful inheritance of cytosine methylation patterns in repeated sequences of the allotetraploid tobacco correlates with the expression of DNA methyltransferase gene families from both parental genomes.Molecular genetics and genomics : MGG · 2009Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic changes accompanying plant cell dedifferentiation and differentiation are reported in 35S ribosomal DNA (rDNA) of tobacco (Nicotiana tabacum). There was a reduction of CG and CNG methylation in both intergenic and genic regions of the rDNA cistron in fully dedifferentiated callus and root compared to leaf. The rDNA hypomethylation was not random, but targeted to particular rDNA gene families at units that are clustered within the tandem array. The process of hypomethylation was initiated as early as 2 weeks after the callus induction and established epigenetic patterns were stably maintained throughout prolonged culture. However, regenerated plants and their progeny showed partial and complete remethylation of units, respectively. Nuclear run-on assays revealed a 2-fold increase of primary (unprocessed) ribosomal RNA transcripts in callus compared to leaf tissue. However, the abundance of mature transcripts in callus was elevated by only about 25%. Fluorescence in situ hybridization analysis of interphase nuclei showed high levels of rDNA chromatin condensation in both callus and leaf, with substantially less decondensed rDNA than is observed in meristematic root-tip cells. It is likely that the regions of the rDNA locus showing decondensation correspond to the clusters of hypomethylated units that occur in the tandem array at each locus. The data together indicate that the establishment of pluripotency and cell proliferation occurring with callus induction is associated with enhanced ribosomal RNA gene expression and overall rDNA hypomethylation, but is not associated with material-enhanced relaxation of chromatin structure (decondensation) at rDNA loci.
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