ArticleBiophysical journal2005
Indentation and adhesive probing of a cell membrane with AFM: theoretical model and experiments.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed, 299 citations in OpenAlex.
- Delamination of epithelia induced by air-liquid interfaces.Molecular biology of the cell · 2025Article
- Targeting ELOVL6 to disrupt c-MYC driven lipid metabolism in pancreatic cancer enhances chemosensitivity.Nature communications · 2025Article
- Size Matters: Rethinking Hertz Model Interpretation for Cell Mechanics Using AFM.International journal of molecular sciences · 2024Article
- Feature Matching of Microsecond-Pulsed Magnetic Fields Combined with FeBiomolecules · 2024Article
- Quantitative Understanding of Ionic Channel Network Variation in Nafion with Hydration Using Current Sensing Atomic Force Microscopy.Polymers · 2024Article
- Tired and stressed: direct holographic quasi-static stretching of aging echinocytes and discocytes in plasma using optical tweezers [Invited].Biomedical optics express · 2024Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Active learning model for extracting elastic modulus of cell on substrate.Biophysical journal · 2023Article
- A reliable elasticity sensing method for analysis of cell entosis using microfluidic cytometer.Biomedical engineering letters · 2023Article
- Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury.Nature communications · 2022Article
- Dynamic alteration of poroelastic attributes as determinant membrane nanorheology for endocytosis of organ specific targeted gold nanoparticles.Journal of nanobiotechnology · 2022Article
- Epithelial cells fluidize upon adhesion but display mechanical homeostasis in the adherent state.Biophysical journal · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Viscoelasticity of basal plasma membranes and cortices derived from MDCK II cells.Biophysical reports · 2021Article
- Superelasticity of Plasma- and Synthetic Membranes Resulting from Coupling of Membrane Asymmetry, Curvature, and Lipid Sorting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Non-contact long-range magnetic stimulation of mechanosensitive ion channels in freely moving animals.Nature materials · 2021Article
- Analysis of Ionic Domains on a Proton Exchange Membrane Using a Numerical Approximation Model Based on Electrostatic Force Microscopy.Polymers · 2021Article
- The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.Science advances · 2021Article
- Nuclear Stiffness Decreases with Disruption of the Extracellular Matrix in Living Tissues.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In probing adhesion and cell mechanics by atomic force microscopy (AFM), the mechanical properties of the membrane have an important if neglected role. Here we theoretically model the contact of an AFM tip with a cell membrane, where direct motivation and data are derived from a prototypical ligand-receptor adhesion experiment. An AFM tip is functionalized with a prototypical ligand, SIRPalpha, and then used to probe its native receptor on red cells, CD47. The interactions prove specific and typical in force, and also show in detachment, a sawtooth-shaped disruption process that can extend over hundreds of nm. The theoretical model here that accounts for both membrane indentation as well as membrane extension in tip retraction incorporates membrane tension and elasticity as well as AFM tip geometry and stochastic disruption. Importantly, indentation depth proves initially proportional to membrane tension and does not follow the standard Hertz model. Computations of detachment confirm nonperiodic disruption with membrane extensions of hundreds of nm set by membrane tension. Membrane mechanical properties thus clearly influence AFM probing of cells, including single molecule adhesion experiments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.