ArticleBiophysical journal2005
Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 45 citations in OpenAlex.
- Heterogeneity of ventricular action potentials in neonatal rat cardiomyocytes and methodological aspects of patch clamp measurements.Frontiers in physiology · 2025Article
- Optically Active, Paper-Based Scaffolds for 3D Cardiac Pacing.ACS applied materials & interfaces · 2024Article
- Circadian regulation of sinoatrial nodal cell pacemaking function: Dissecting the roles of autonomic control, body temperature, and local circadian rhythmicity.PLoS computational biology · 2024Article
- ThermoCyte: an inexpensive open-source temperature control system forRoyal Society open science · 2023Article
- Local Control Model of a Human Ventricular Myocyte: An Exploration of Frequency-Dependent Changes and Calcium Sparks.Biomolecules · 2023Article
- Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor.Sensors (Basel, Switzerland) · 2023Article
- Article
- Thermal modulation of epicardial Ca2+ dynamics uncovers molecular mechanisms of Ca2+ alternans.The Journal of general physiology · 2021Article
- Ischemia Enhances the Acute Stretch-Induced Increase in Calcium Spark Rate in Ventricular Myocytes.Frontiers in physiology · 2020Article
- Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels.Frontiers in physiology · 2020Article
- Pulsed infrared releases CaJournal of neurophysiology · 2018Article
- Interaction between neuronal nitric oxide synthase signaling and temperature influences sarcoplasmic reticulum calcium leak: role of nitroso-redox balance.Circulation research · 2015Article
- Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells.Heart and vessels · 2014Article
- Calcium waves initiating from the anomalous subdiffusive calcium sparks.Journal of the Royal Society, Interface · 2014Article
- Temperature and transmural region influence functional measurements in unloaded left ventricular cardiomyocytes.Physiological reports · 2013Article
- Fluorescent tag is not a reliable marker for small RNA transfection in the presence of serum.Journal of biosciences · 2013Article
- Temperature dependence of IP3-mediated local and global Ca2+ signals.Biophysical journal · 2013Article
- Ultrastructural remodelling of Ca(2+) signalling apparatus in failing heart cells.Cardiovascular research · 2012Article
- Ca2+ cycling in heart cells from ground squirrels: adaptive strategies for intracellular Ca2+ homeostasis.PloS one · 2011Article
- Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.Biomedical optics express · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the temperature dependence and underlying thermodynamic determinants of the elementary Ca2+ release from the sarcoplasmic reticulum, we characterized Ca2+ sparks originating from ryanodine receptors (RyRs) in rat cardiomyocytes over a wide range of temperature. From 35 degrees C to 10 degrees C, the normalized fluo-3 fluorescence of Ca2+ sparks decreased monotonically, but the Delta[Ca2+]i were relatively unchanged due to increased resting [Ca2+]i. The time-to-peak of Ca2+ sparks, which represents the RyR Ca2+ release duration, was prolonged by 37% from 35 degrees C to 10 degrees C. An Arrhenius plot of the data identified a jump of apparent activation energy from 5.2 to 14.6 kJ/mol at 24.8 degrees C, which presumably reflects a transition of sarcoplasmic reticulum lipids. Thermodynamic analysis of the decay kinetics showed that active transport plays little role in early recovery but a significant role in late recovery of local Ca2+ concentration. These results provided a basis for quantitative interpretation of intracellular Ca2+ signaling under various thermal conditions. The relative temperature insensitivity above the transitional 25 degrees C led to the notion that Ca2+ sparks measured at a "warm room" temperature are basically acceptable in elucidating mammalian heart function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.