ArticleBiophysical journal2005
Probing vesicle dynamics in single hippocampal synapses.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
- The nanoscale mobility of calcium channels is driven by readily releasable synaptic vesicles to support precise neurotransmission in livebioRxiv : the preprint server for biology · 2026Article
- Initial Distance-Dependent Mean Force Drives Synaptic Vesicle Motion Toward Fusion Sites in Stimulated Hippocampal Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Optical Approaches to Dissect the Structure and Dynamics of the Synapse at Nanoscale Resolution.Advances in neurobiology · 2026Review
- Actomyosin-mediated inhibition of synaptic vesicle release under CBTranslational psychiatry · 2024Article
- Statistical analysis of the autocorrelation function in fluorescence correlation spectroscopy.Biophysical journal · 2024Article
- Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles.Nature communications · 2024Article
- A comparative analysis of the mobility of 45 proteins in the synaptic bouton.The EMBO journal · 2020Article
- Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules.Nature protocols · 2017Article
- Activity-Dependence of Synaptic Vesicle Dynamics.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses.eLife · 2017Article
- Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles.The Journal of cell biology · 2016Article
- Article
- Calmodulin enhances ribbon replenishment and shapes filtering of synaptic transmission by cone photoreceptors.The Journal of general physiology · 2014Article
- Temperature dependence of vesicular dynamics at excitatory synapses of rat hippocampus.Cognitive neurodynamics · 2014Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Is replenishment of the readily releasable pool associated with vesicular movement?Cognitive neurodynamics · 2014Article
- Synaptic bouton properties are tuned to best fit the prevailing firing pattern.Frontiers in computational neuroscience · 2014Article
- Recovery of vesicular storage and release parameters after high frequency stimulation in rat hippocampus.Cognitive neurodynamics · 2013Article
- Calcium, calpain, and calcineurin in low-frequency depression of transmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Synaptic vesicle pools and dynamics.Cold Spring Harbor perspectives in biology · 2012Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicles is mobile, taking seconds to traverse the synapse. Applying the phosphatase inhibitor okadaic acid causes vesicles to diffuse freely, moving 30 times faster than vesicles in control synapses. These results suggest that vesicles move sluggishly due to binding to elements of the synaptic cytomatrix and that this binding is altered by phosphorylation. Motivated by these results, a model is constructed consisting of diffusing vesicles that bind reversibly to the cytomatrix. This stick-and-diffuse model accounts for the fluorescence correlation spectroscopy and fluorescence recovery after photobleaching data, and also predicts the well-known exponential refilling of the readily releasable pool. Our measurements suggest that the movement of vesicles to the active zone is the rate-limiting step in this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.