ArticlePLoS biology2005
The genomics of disulfide bonding and protein stabilization in thermophiles.
Article in PLoS biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 198 citations in OpenAlex.
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- Neural network conditioned to produce thermophilic protein sequences can increase thermal stability.Scientific reports · 2025Article
- Comparative analysis of thermal adaptations of extremophilic prolyl oligopeptidases.Biophysical journal · 2024Article
- Comparative Modeling and Analysis of Extremophilic D-Ala-D-Ala Carboxypeptidases.Biomolecules · 2023Article
- Thermal Endurance by a Hot-Spring-Dwelling Phylogenetic Relative of the MesophilicMicrobiology spectrum · 2022Article
- A Comparative Analysis ofInternational journal of molecular sciences · 2022Article
- In Silico Analysis of Glucose Oxidase fromBioengineering (Basel, Switzerland) · 2021Article
- Heat adaptation of phage T7 under an extended genetic code.Virus evolution · 2021Article
- Improving the catalytic efficiency and substrate affinity of a novel esterase from marine Klebsiella aerogenes by random and site-directed mutation.World journal of microbiology & biotechnology · 2021Article
- A novel hyperthermophilic methylglyoxal synthase: molecular dynamic analysis on the regional fluctuations.Scientific reports · 2021Article
- Comparative Genome Analysis ofMicroorganisms · 2020Article
- Enzymatic Antioxidant Signatures in Hyperthermophilic Archaea.Antioxidants (Basel, Switzerland) · 2020Review
- Multi-omics reveals mechanisms of total resistance to extreme illumination of a desert alga.Nature plants · 2020Article
- The structures of two archaeal type IV pili illuminate evolutionary relationships.Nature communications · 2020Article
- High-throughput quantification of protein structural change reveals potential mechanisms of temperature adaptation in Mytilus mussels.BMC evolutionary biology · 2020Article
- Comparative Genomic Analysis Reveals the Metabolism and Evolution of the Thermophilic Archaeal GenusFrontiers in microbiology · 2020Article
- Stabilizing HIV-1 envelope glycoprotein trimers to induce neutralizing antibodies.Retrovirology · 2018Review
29 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Thermophilic organisms flourish in varied high-temperature environmental niches that are deadly to other organisms. Recently, genomic evidence has implicated a critical role for disulfide bonds in the structural stabilization of intracellular proteins from certain of these organisms, contrary to the conventional view that structural disulfide bonds are exclusively extracellular. Here both computational and structural data are presented to explore the occurrence of disulfide bonds as a protein-stabilization method across many thermophilic prokaryotes. Based on computational studies, disulfide-bond richness is found to be widespread, with thermophiles containing the highest levels. Interestingly, only a distinct subset of thermophiles exhibit this property. A computational search for proteins matching this target phylogenetic profile singles out a specific protein, known as protein disulfide oxidoreductase, as a potential key player in thermophilic intracellular disulfide-bond formation. Finally, biochemical support in the form of a new crystal structure of a thermophilic protein with three disulfide bonds is presented together with a survey of known structures from the literature. Together, the results provide insight into biochemical specialization and the diversity of methods employed by organisms to stabilize their proteins in exotic environments. The findings also motivate continued efforts to sequence genomes from divergent organisms.
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