ArticleJournal of bacteriology2005
Heterocyst-specific excision of the Anabaena sp. strain PCC 7120 hupL element requires xisC.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Predicting FOX gene candidates for oxic nitrogen fixation using multi-omic machine learning and comparative bioinformatics.Scientific reports · 2026Article
- Restoration of the Functional nif Gene Cluster by Complex Recombination Events during Heterocyst Development in the Nitrogen-Fixing Cyanobacterium Calothrix sp. NIES-4101.Plant & cell physiology · 2024Article
- Photobiohydrogen Production and Strategies for HAdvances in biochemical engineering/biotechnology · 2023Article
- Article
- ϕSa3mw Prophage as a Molecular Regulatory Switch of Staphylococcus aureus β-Toxin Production.Journal of bacteriology · 2019Article
- CRISPR-Cas systems in multicellular cyanobacteria.RNA biology · 2019Article
- Genomic Characteristics ofFrontiers in microbiology · 2019Article
- Article
- A new perspective on lysogeny: prophages as active regulatory switches of bacteria.Nature reviews. Microbiology · 2015Review
- How close we are to achieving commercially viable large-scale photobiological hydrogen production by cyanobacteria: a review of the biological aspects.Life (Basel, Switzerland) · 2015Review
- Bacterial genome instability.Microbiology and molecular biology reviews : MMBR · 2014Review
- Mobile elements in a single-filament orange Guaymas Basin Beggiatoa ("Candidatus Maribeggiatoa") sp. draft genome: evidence for genetic exchange with cyanobacteria.Applied and environmental microbiology · 2013Article
- Article
- Expression of Shewanella oneidensis MR-1 [FeFe]-hydrogenase genes in Anabaena sp. strain PCC 7120.Applied and environmental microbiology · 2012Article
- Inactivation of uptake hydrogenase leads to enhanced and sustained hydrogen production with high nitrogenase activity under high light exposure in the cyanobacterium Anabaena siamensis TISTR 8012.Journal of biological engineering · 2012Article
- Genetic engineering of cyanobacteria to enhance biohydrogen production from sunlight and water.Ambio · 2012Article
- The sigE gene is required for normal expression of heterocyst-specific genes in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2011Article
- Nitrogen fixation and hydrogen metabolism in cyanobacteria.Microbiology and molecular biology reviews : MMBR · 2010Review
- Cyanobacterial heterocysts.Cold Spring Harbor perspectives in biology · 2010Review
- Transcription of hupSL in Anabaena variabilis ATCC 29413 is regulated by NtcA and not by hydrogen.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
In nitrogen-limiting conditions, approximately 10% of the vegetative cells in filaments of the cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 differentiate into nitrogen-fixing heterocysts. During the late stages of heterocyst differentiation, three DNA elements, each embedded within an open reading frame, are programmed to excise from the chromosome by site-specific recombination. The DNA elements are named after the genes that they interrupt: nifD, fdxN, and hupL. The nifD and fdxN elements each contain a gene, xisA or xisF, respectively, that encodes the site-specific recombinase required for programmed excision of the element. Here, we show that the xisC gene (alr0677), which is present at one end of the 9,435-bp hupL element, is required for excision of the hupL element. A strain in which the xisC gene was inactivated showed no detectable excision of the hupL element. hupL encodes the large subunit of uptake hydrogenase. The xisC mutant forms heterocysts and grows diazotrophically, but unlike the wild type, it evolved hydrogen gas under nitrogen-fixing conditions. Overexpression of xisC from a plasmid in a wild-type background caused a low level of hupL rearrangement even in nitrogen-replete conditions. Expression of xisC in Escherichia coli was sufficient to produce rearrangement of an artificial substrate plasmid bearing the hupL element recombination sites. Sequence analysis indicated that XisC is a divergent member of the phage integrase family of recombinases. Site-directed mutagenesis of xisC showed that the XisC recombinase has functional similarity to the phage integrase family.
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