ArticleJournal of bacteriology2005
E1 enzyme of the pyruvate dehydrogenase complex in Corynebacterium glutamicum: molecular analysis of the gene and phylogenetic aspects.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 83 citations in OpenAlex.
- Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation.Microbial cell factories · 2025Article
- Cellular localization of the hybrid pyruvate/2-oxoglutarate dehydrogenase complex in the actinobacteriumMicrobiology spectrum · 2023Article
- Article
- Identification of Differentially Expressed Genes in Nocardia brasiliensis Induced by Progesterone and Dihydrotestosterone Using Differential Display PCR.Current microbiology · 2022Article
- CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production byMicroorganisms · 2021Article
- A Timed Off-Switch for Dynamic Control of Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Disruption of the Oxidative Pentose Phosphate Pathway Stimulates High-Yield Production Using Resting Corynebacterium glutamicum in the Absence of External Electron Acceptors.Applied and environmental microbiology · 2020Article
- Inducible Expression Systems Based on Xenogeneic Silencing and Counter-Silencing and Design of a Metabolic Toggle Switch.ACS synthetic biology · 2020Article
- The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis.BMC microbiology · 2020Article
- Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.Metabolic engineering · 2020Article
- The Iron Deficiency Response of Corynebacterium glutamicum and a Link to Thiamine Biosynthesis.Applied and environmental microbiology · 2020Article
- Impact of COJournal of bacteriology · 2019Article
- One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst.Scientific reports · 2018Article
- Challenges of ligand identification for the second wave of orphan riboswitch candidates.RNA biology · 2018Article
- Comparative proteomic analysis of outer membrane protein 43 (Journal of veterinary science · 2018Article
- A novel aceE mutation leading to a better growth profile and a higher L-serine production in a high-yield L-serine-producing Corynebacterium glutamicum strain.Journal of industrial microbiology & biotechnology · 2016Article
- 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.Molecular biotechnology · 2016Article
- Engineering Corynebacterium glutamicum for the production of 2,3-butanediol.Microbial cell factories · 2015Article
- The α-glucan phosphorylase MalP of Corynebacterium glutamicum is subject to transcriptional regulation and competitive inhibition by ADP-glucose.Journal of bacteriology · 2015Article
- Pan-genome analyses identify lineage- and niche-specific markers of evolution and adaptation in Epsilonproteobacteria.Frontiers in microbiology · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The E1p enzyme is an essential part of the pyruvate dehydrogenase complex (PDHC) and catalyzes the oxidative decarboxylation of pyruvate with concomitant acetylation of the E2p enzyme within the complex. We analyzed the Corynebacterium glutamicum aceE gene, encoding the E1p enzyme, and constructed and characterized an E1p-deficient mutant. Sequence analysis of the C. glutamicum aceE gene and adjacent regions revealed that aceE is not flanked by genes encoding other enzymes of the PDHC. Transcriptional analysis revealed that aceE from C. glutamicum is monocistronic and that its transcription is initiated 121 nucleotides upstream of the translational start site. Inactivation of the chromosomal aceE gene led to the inability to grow on glucose and to the absence of PDHC and E1p activities, indicating that only a single E1p enzyme is present in C. glutamicum and that the PDHC is essential for the growth of this organism on carbohydrate substrates. Surprisingly, the E1p enzyme of C. glutamicum showed up to 51% identity to homodimeric E1p proteins from gram-negative bacteria but no similarity to E1 alpha- or beta-subunits of heterotetrameric E1p enzymes which are generally assumed to be typical for gram-positives. To investigate the distribution of E1p enzymes in bacteria, we compiled and analyzed the phylogeny of 46 homodimeric E1p proteins and of 58 alpha-subunits of heterotetrameric E1p proteins deposited in public databases. The results revealed that the distribution of homodimeric and heterotetrameric E1p subunits in bacteria is not in accordance with the rRNA-based phylogeny of bacteria and is more heterogeneous than previously assumed.
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