ArticleJournal of bacteriology2005
The heat shock genes dnaK, dnaJ, and grpE are involved in regulation of putisolvin biosynthesis in Pseudomonas putida PCL1445.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
- Lipopeptide Engineering: From Natural Origins to Rational Design Against Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2026Review
- Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5.Environmental microbiology reports · 2025Article
- Does regulation hold the key to optimizing lipopeptide production inFrontiers in bioengineering and biotechnology · 2024Review
- Article
- Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors.Engineering microbiology · 2023Review
- Cytoplasmic molecular chaperones in Pseudomonas species.Journal of microbiology (Seoul, Korea) · 2022Review
- DnaJ and ClpX Are Required for HitRS and HssRS Two-Component System Signaling in Bacillus anthracis.Infection and immunity · 2022Article
- High-throughput identification of genes influencing the competitive ability to obtain nutrients and performance of biocontrol in Pseudomonas putida JBC17.Scientific reports · 2022Article
- Development and characterization of efficient xylose utilization strains of Zymomonas mobilis.Biotechnology for biofuels · 2021Article
- Article
- Biosurfactants: Potential Agents for Controlling Cellular Communication, Motility, and Antagonism.Frontiers in molecular biosciences · 2021Review
- LeveragingFrontiers in microbiology · 2021Review
- Microbial Culture in Minimal Medium With Oil Favors Enrichment of Biosurfactant Producing Genes.Frontiers in bioengineering and biotechnology · 2020Article
- Comparative Genomics Reveals Ecological and Evolutionary Insights into Sponge-AssociatedmSystems · 2019Article
- Dual Gene Expression Analysis Identifies Factors Associated with Staphylococcus aureus Virulence in Diabetic Mice.Infection and immunity · 2019Article
- Temporal proteomic profiling reveals changes that support Burkholderia biofilms.Pathogens and disease · 2019Article
- The LuxR Regulators PcoR and RfiA Co-regulate Antimicrobial Peptide and Alginate Production inFrontiers in microbiology · 2018Article
- Biosynthesis of the antimicrobial cyclic lipopeptides nunamycin and nunapeptin by Pseudomonas fluorescens strain In5 is regulated by the LuxR-type transcriptional regulator NunF.MicrobiologyOpen · 2017Article
- Coregulation of the cyclic lipopeptides orfamide and sessilin in the biocontrol strain Pseudomonas sp. CMR12a.MicrobiologyOpen · 2017Article
- Genetic Dissection of the Regulatory Network Associated with High c-di-GMP Levels in Pseudomonas putida KT2440.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida PCL1445 produces two cyclic lipopeptides, putisolvins I and II, which possess surfactant activity and play an important role in biofilm formation and degradation. In order to identify genes and traits that are involved in the regulation of putisolvin production of PCL1445, a Tn5luxAB library was generated and mutants were selected for the lack of biosurfactant production using a drop-collapsing assay. Sequence analysis of the Tn5luxAB flanking region of one biosurfactant mutant, strain PCL1627, showed that the transposon had inserted in a dnaK homologue which is located downstream of grpE and upstream of dnaJ. Analysis of putisolvin production and expression studies indicate that dnaK, together with the dnaJ and grpE heat shock genes, takes part in the positive regulation (directly or indirectly) of putisolvin biosynthesis at the transcriptional level. Growth of PCL1445 at low temperature resulted in an increased level of putisolvins, and mutant analyses showed that this requires dnaK and dnaJ but not grpE. In addition, putisolvin biosynthesis of PCL1445 was found to be dependent on the GacA/GacS two-component signaling system. Expression analysis indicated that dnaK is positively regulated by GacA/GacS.
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