ArticleJournal of bacteriology2005
A phylogeny of bacterial RNA nucleotidyltransferases: Bacillus halodurans contains two tRNA nucleotidyltransferases.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Crystal structure of the CCA-adding enzyme fromJournal of structural biology: X · 2025Article
- CCA-Addition Gone Wild: Unusual Occurrence and Phylogeny of Four Different tRNA Nucleotidyltransferases in Acanthamoeba castellanii.Molecular biology and evolution · 2021Article
- Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes inInternational journal of molecular sciences · 2020Article
- Divergent Evolution of Eukaryotic CC- and A-Adding Enzymes.International journal of molecular sciences · 2020Article
- Phylogeny and Evolution of RNA 3'-Nucleotidyltransferases in Bacteria.Journal of molecular evolution · 2019Article
- Dual expression of CCA-adding enzyme and RNase T in Escherichia coli generates a distinct cca growth phenotype with diverse applications.Nucleic acids research · 2019Article
- Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases.Nucleic acids research · 2016Article
- Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases.Frontiers in genetics · 2014Review
- An inhibitory C-terminal region dictates the specificity of A-adding enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the hyperthermophilic nucleotidyltransferase TTHA1015 from Thermus thermophilus HB8.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Unusual evolution of a catalytic core element in CCA-adding enzymes.Nucleic acids research · 2010Article
- tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.Cellular and molecular life sciences : CMLS · 2010Review
- CCA addition to tRNA: implications for tRNA quality control.IUBMB life · 2010Review
- Mechanism for the definition of elongation and termination by the class II CCA-adding enzyme.The EMBO journal · 2009Article
- Geobacter sulfurreducens contains separate C- and A-adding tRNA nucleotidyltransferases and a poly(A) polymerase.Journal of bacteriology · 2009Article
- A comparative analysis of two conserved motifs in bacterial poly(A) polymerase and CCA-adding enzyme.Nucleic acids research · 2008Article
- Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme.The EMBO journal · 2008Article
- Evolution of tRNA nucleotidyltransferases: a small deletion generated CC-adding enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Reengineering CCA-adding enzymes to function as (U,G)- or dCdCdA-adding enzymes or poly(C,A) and poly(U,G) polymerases.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of several bacterial species encode only one member of the nucleotidyltransferase superfamily (NTSF), and if that protein functions as a TNT, those organisms may not contain a poly(A) polymerase I like that of Escherichia coli. The genomes of several of the species examined encode more than one member of the nucleotidyltransferase superfamily. The function of some of those proteins is known, but in most cases no biochemical activity has been assigned to the NTSF. The NTSF protein sequences were used to construct an unrooted phylogenetic tree. To learn more about the function of the NTSFs in species whose genomes encode more than one, we have examined Bacillus halodurans. We have demonstrated that B. halodurans adds poly(A) tails to the 3' ends of RNAs in vivo. We have shown that the genes for both of the NTSFs encoded by the B. halodurans genome are transcribed in vivo. We have cloned, overexpressed, and purified the two NTSFs and have shown that neither functions as poly(A) polymerase in vitro. Rather, the two proteins function as tRNA nucleotidyltransferases, and our data suggest that, like some of the deep branching bacterial species previously studied by others, B. halodurans possesses separate CC- and A-adding tRNA nucleotidyltransferases. These observations raise the interesting question of the identity of the enzyme responsible for RNA polyadenylation in Bacillus.
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