ArticleJournal of bacteriology2005
ABC transporter for corrinoids in Halobacterium sp. strain NRC-1.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Computational Strategies to Enhance Vitamin BCurrent microbiology · 2025Review
- Corrinoid salvaging and cobamide remodeling in bacteria and archaea.Journal of bacteriology · 2024Review
- Comparative Genomics ofMicroorganisms · 2023Article
- Expanded Diversity and Metabolic Versatility of Marine Nitrite-Oxidizing Bacteria Revealed by Cultivation- and Genomics-Based Approaches.Applied and environmental microbiology · 2020Article
- Genomic and transcriptomic evidence of light-sensing, porphyrin biosynthesis, Calvin-Benson-Bassham cycle, and urea production in Bathyarchaeota.Microbiome · 2020Article
- The Methanosarcina mazei MM2060 Gene Encodes a Bifunctional Kinase/Decarboxylase Enzyme Involved in Cobamide Biosynthesis.Biochemistry · 2018Article
- Underlying mechanisms for syntrophic metabolism of essential enzyme cofactors in microbial communities.The ISME journal · 2017Article
- Microbial production of vitamin BMicrobial cell factories · 2017Review
- Whole genomic sequence analysis of Bacillus infantis: defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe.BMC genomics · 2016Article
- Microbial ecology of an Antarctic hypersaline lake: genomic assessment of ecophysiology among dominant haloarchaea.The ISME journal · 2014Article
- Thermotoga lettingae can salvage cobinamide to synthesize vitamin B12.Applied and environmental microbiology · 2013Article
- Genome-wide responses of the model archaeon Halobacterium sp. strain NRC-1 to oxygen limitation.Journal of bacteriology · 2012Article
- Vitamin B(12) synthesis and salvage pathways were acquired by horizontal gene transfer to the Thermotogales.Genome biology and evolution · 2012Article
- An ABC-type cobalt transport system is essential for growth of Sinorhizobium meliloti at trace metal concentrations.Journal of bacteriology · 2011Article
- The cobinamide amidohydrolase (cobyric acid-forming) CbiZ enzyme: a critical activity of the cobamide remodelling system of Rhodobacter sphaeroides.Molecular microbiology · 2009Article
- Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii.Biochemistry · 2009Article
- In vivo analysis of cobinamide salvaging in Rhodobacter sphaeroides strain 2.4.1.Journal of bacteriology · 2009Article
- Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA.BMC genetics · 2007Article
- Conversion of cobinamide into adenosylcobamide in bacteria and archaea.Journal of bacteriology · 2007Review
- The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We report evidence for the existence of a putative ABC transporter for corrinoid utilization in the extremely halophilic archaeon Halobacterium sp. strain NRC-1. Results from genetic and nutritional analyses of Halobacterium showed that mutants with lesions in open reading frames (ORFs) Vng1370G, Vng1371Gm, and Vng1369G required a 10(5)-fold higher concentration of cobalamin for growth than the wild-type or parent strain. The data support the conclusion that these ORFs encode orthologs of the bacterial cobalamin ABC transporter permease (btuC; Vng1370G), ATPase (btuD; Vng1371Gm), and substrate-binding protein (btuF; Vng1369G) components. Mutations in the Vng1370G, Vng1371Gm, and Vng1369G genes were epistatic, consistent with the hypothesis that their products work together to accomplish the same function. Extracts of btuF mutant strains grown in the presence of cobalamin did not contain any cobalamin molecules detectable by a sensitive bioassay, whereas btuCD mutant strain extracts did. The data are consistent with the hypothesis that the BtuF protein is exported to the extracellular side of the cell membrane, where it can bind cobalamin in the absence of BtuC and BtuD. Our data also provide evidence for the regulation of corrinoid transport and biosynthesis. Halobacterium synthesized cobalamin in a chemically defined medium lacking corrinoid precursors. To the best of our knowledge, this is the first genetic analysis of an archaeal corrinoid transport system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.