ArticleFree radical biology & medicine2005
Endogenous DNA damage clusters in human skin, 3-D model, and cultured skin cells.
Article in Free radical biology & medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams.International journal of molecular sciences · 2023Article
- 8-Oxoguanine: from oxidative damage to epigenetic and epitranscriptional modification.Experimental & molecular medicine · 2022Review
- Evaluation of Histone Deacetylase Inhibitors as Radiosensitizers for Proton and Light Ion Radiotherapy.Frontiers in oncology · 2021Article
- Clustered abasic lesions profoundly change the structure and stability of human telomeric G-quadruplexes.Nucleic acids research · 2017Article
- Comments on potential health effects of MRI-induced DNA lesions: quality is more important to consider than quantity.European heart journal. Cardiovascular Imaging · 2016Review
- Reduced repair capacity of a DNA clustered damage site comprised of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 2-deoxyribonolactone results in an increased mutagenic potential of these lesions.Mutation research · 2014Article
- Article
- Clustered DNA lesion repair in eukaryotes: relevance to mutagenesis and cell survival.Mutation research · 2011Review
- Oxidative DNA damage is a preliminary step during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide.Journal of molecular histology · 2011Article
- Processing of abasic DNA clusters in hApeI-silenced primary fibroblasts exposed to low doses of X-irradiation.Journal of biosciences · 2011Article
- Abasic sites preferentially form at regions undergoing DNA replication.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Accumulation of oxidatively induced clustered DNA lesions in human tumor tissues.Mutation research · 2009Article
- Human abasic endonuclease action on multilesion abasic clusters: implications for radiation-induced biological damage.Nucleic acids research · 2008Article
- Low levels of clustered oxidative DNA damage induced at low and high LET irradiation in mammalian cells.Radiation and environmental biophysics · 2006Article
- Role of oxidatively induced DNA lesions in human pathogenesis.Mutation researchReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Clustered damages-two or more oxidized bases, abasic sites, or strand breaks on opposing DNA strands within a few helical turns-are formed in DNA by ionizing radiation. Clusters are difficult for cells to repair and thus pose significant challenges to genomic integrity. Although endogenous clusters were found in some permanent human cell lines, it was not known if clusters accumulated in human tissues or primary cells. Using high-sensitivity gel electrophoresis, electronic imaging, and number average length analysis, we determined endogenous cluster levels in DNA from human skin, a 3-D skin model, and primary cultured skin cells. DNA from dermis and epidermis of human skin contained extremely low levels of endogenous clusters (a few per gigabase). However, cultured skin fibroblasts and keratinocytes-whether in monolayer cultures or in 3-D model skin cultures-accumulated oxidized pyrimidine, oxidized purine, and abasic clusters. The levels of endogenous clusters were decreased by growing cells in the presence of selenium or by increasing cellular levels of Fpg protein, presumably by increasing processing of clustered damages. These results imply that the levels of endogenous clusters can be affected by the cells' external environment and their ability to deal with DNA damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.