ArticleBMC evolutionary biology2005
An ancient spliceosomal intron in the ribosomal protein L7a gene (Rpl7a) of Giardia lamblia.
Article in BMC evolutionary biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed.
- Spliceosomal introns in the diplomonad parasiteMicrobial genomics · 2023Article
- Analysis of Fungal Genomes Reveals Commonalities of Intron Gain or Loss and Functions in Intron-Poor Species.Molecular biology and evolution · 2021Article
- The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.PLoS neglected tropical diseases · 2021Article
- Article
- Patterns of conservation of spliceosomal intron structures and spliceosome divergence in representatives of the diplomonad and parabasalid lineages.BMC evolutionary biology · 2019Article
- Sense-antisense gene overlap is probably a cause for retaining the few introns in Giardia genome and the implications.Biology direct · 2018Article
- The Putative Smallest Introns in the Arabidopsis Genome.Genome biology and evolution · 2018Article
- Interactions between Giardia duodenalis Sm proteins and their association with spliceosomal snRNAs.Parasitology research · 2017Article
- Transcriptomic analysis of diplomonad parasites reveals a trans-spliced intron in a helicase gene inPeerJ · 2017Article
- Secondary loss of a cis-spliced intron during the divergence of Giardia intestinalis assemblages.BMC research notes · 2014Article
- Transcriptome profiling of Giardia intestinalis using strand-specific RNA-seq.PLoS computational biology · 2013Article
- Evolutionarily divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia.Nucleic acids research · 2012Article
- Origin and evolution of spliceosomal introns.Biology direct · 2012Review
- Widespread recurrent evolution of genomic features.Genome biology and evolution · 2012Review
- A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia.RNA (New York, N.Y.) · 2011Article
- U12 type introns were lost at multiple occasions during evolution.BMC genomics · 2010Article
- Computational identification of four spliceosomal snRNAs from the deep-branching eukaryote Giardia intestinalis.PloS one · 2008Article
- Computational screen for spliceosomal RNA genes aids in defining the phylogenetic distribution of major and minor spliceosomal components.Nucleic acids research · 2008Article
- Patterns of intron gain and conservation in eukaryotic genes.BMC evolutionary biology · 2007Article
- Evolutionarily conserved genes preferentially accumulate introns.Genome research · 2007Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundOnly one spliceosomal-type intron has previously been identified in the unicellular eukaryotic parasite, Giardia lamblia (a diplomonad). This intron is only 35 nucleotides in length and is unusual in possessing a non-canonical 5' intron boundary sequence, CT, instead of GT.
resultsWe have identified a second spliceosomal-type intron in G. lamblia, in the ribosomal protein L7a gene (Rpl7a), that possesses a canonical GT 5' intron boundary sequence. A comparison of the two known Giardia intron sequences revealed extensive nucleotide identity at both the 5' and 3' intron boundaries, similar to the conserved sequence motifs recently identified at the boundaries of spliceosomal-type introns in Trichomonas vaginalis (a parabasalid). Based on these observations, we searched the partial G. lamblia genome sequence for these conserved features and identified a third spliceosomal intron, in an unassigned open reading frame. Our comprehensive analysis of the Rpl7a intron in other eukaryotic taxa demonstrates that it is evolutionarily conserved and is an ancient eukaryotic intron.
conclusionAn analysis of the phylogenetic distribution and properties of the Rpl7a intron suggests its utility as a phylogenetic marker to evaluate particular eukaryotic groupings. Additionally, analysis of the G. lamblia introns has provided further insight into some of the conserved and unique features possessed by the recently identified spliceosomal introns in related organisms such as T. vaginalis and Carpediemonas membranifera.
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