ArticleMolecular and cellular biology2005
ELAV multimerizes on conserved AU4-6 motifs important for ewg splicing regulation.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Electrophoretic Mobility Shift Assay (EMSA) for Assessing RNA-Protein Binding and Complex Formation Using Recombinant RNA-Binding Proteins and In Vitro-Transcribed RNA.Bio-protocol · 2026Article
- Article
- Angel wing: a class of alternative splicing regulators in animals.Nucleic acids research · 2025Article
- Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3' processing.The EMBO journal · 2025Article
- Phylogenomic instructed target analysis reveals ELAV complex binding to multiple optimally spaced U-rich motifs.Nucleic acids research · 2024Article
- HPF1 regulates tendon stem/progenitor cell senescence and tendon repair via PARP1-mediated poly-ADP ribosylation of HuR.Genes & genomics · 2024Article
- Memory consolidation in honey bees is enhanced by down-regulation ofFrontiers in molecular neuroscience · 2023Article
- Hu Antigen R (HuR) Protein Structure, Function and Regulation in Hepatobiliary Tumors.Cancers · 2022Review
- Regulation of the Alternative Neural Transcriptome by ELAV/Hu RNA Binding Proteins.Frontiers in genetics · 2022Review
- Repression of the Hox gene abd-A by ELAV-mediated Transcriptional Interference.PLoS genetics · 2021Article
- Dynamically expressed single ELAV/Hu orthologue elavl2 of bees is required for learning and memory.Communications biology · 2021Article
- ELAV/Hu RNA binding proteins determine multiple programs of neural alternative splicing.PLoS genetics · 2021Article
- Poly(ADP-ribosyl)ation enhances HuR oligomerization and contributes to pro-inflammatory gene mRNA stabilization.Cellular and molecular life sciences : CMLS · 2021Article
- Overlapping Activities of ELAV/Hu Family RNA Binding Proteins Specify the Extended Neuronal 3' UTR Landscape in Drosophila.Molecular cell · 2020Article
- Acute thiamethoxam toxicity in honeybees is not enhanced by common fungicide and herbicide and lacks stress-induced changes in mRNA splicing.Scientific reports · 2019Article
- HuR biological function involves RRM3-mediated dimerization and RNA binding by all three RRMs.Nucleic acids research · 2019Article
- Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP.Scientific reports · 2017Article
- mNature · 2016Article
- Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.Molecular and cellular biology · 2015Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
ELAV is a gene-specific regulator of alternative pre-mRNA processing in Drosophila neurons. Since ELAV/Hu proteins preferentially bind to AU-rich regions that are generally abundant in introns and untranslated regions, it has not been clear how gene specificity is achieved. Here we used a combination of in vitro biochemical experiments together with phylogenetic comparisons and in vivo analysis of Drosophila transgenes to study ELAV binding to the last ewg intron and splicing regulation. In vitro binding studies of ELAV show that ELAV multimerizes on the ewg binding site and forms a defined and saturable complex. Further, sizing of the ELAV-RNA complex and a series of titration experiments indicate that ELAV forms a dodecameric complex on 135 nucleotides in the last ewg intron. Analysis of the substrate RNA requirements for ELAV binding and complex formation indicates that a series of AU(4-6) motifs spread over the entire binding site are important, but not a strictly defined sequence element. The importance of AU(4-6) motifs, but not spacing between them, is further supported by evolutionary conservation in several melanogaster species subgroups. Finally, using transgenes we demonstrate in fly neurons that ELAV-mediated regulation of ewg intron 6 splicing requires several AU(4-6) motifs and that introduction of spacer sequence between conserved AU(4-6) motifs has a minimal effect on splicing. Collectively, our results suggest that ELAV multimerization and binding to multiple AU(4-6) motifs contribute to target RNA recognition and processing in a complex cellular environment.
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