ArticleGut2006
Serum from achalasia patients alters neurochemical coding in the myenteric plexus and nitric oxide mediated motor response in normal human fundus.
Article in Gut, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- Idiopathic achalasia: unraveling the etiology and pathogenesis of an inflammatory disease.Gastroenterology report · 2026Review
- Achalasia.Nature reviews. Disease primers · 2022Review
- Cardiovascular dysautonomia in Achalasia Patients: Blood pressure and heart rate variability alterations.PloS one · 2021Article
- Neuronal Control of Esophageal Peristalsis and Its Role in Esophageal Disease.Current gastroenterology reports · 2019Review
- Esophageal motility disorders: new perspectives from high-resolution manometry and histopathology.Journal of gastroenterology · 2018Review
- An Overview of Achalasia and Its Subtypes.Gastroenterology & hepatology · 2017Article
- New insights into the pathophysiology of achalasia and implications for future treatment.World journal of gastroenterology · 2016Review
- Gene expression of muscular and neuronal pathways is cooperatively dysregulated in patients with idiopathic achalasia.Scientific reports · 2016Article
- Achalasia--An Autoimmune Inflammatory Disease: A Cross-Sectional Study.Journal of immunology research · 2015Observational
- Gastrointestinal motility disorders in inflammatory bowel diseases.World journal of gastroenterology · 2014Review
- The spectrum of achalasia: lessons from studies of pathophysiology and high-resolution manometry.Gastroenterology · 2013Review
- Chronic constipation: no more idiopathic, but a true neuropathological entity.Gastroenterology and hepatology from bed to bench · 2011Review
- New techniques in the tissue diagnosis of gastrointestinal neuromuscular diseases.World journal of gastroenterology · 2009Review
- Article
- Article
- Impact of high-resolution manometry on achalasia diagnosis and treatment.Annals of gastroenterologyReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAchalasia is a disease of unknown aetiology. An immune mechanism has been suggested on the basis of previous morphological observations. The objective of this study was to test whether the serum of achalasia patients could reproduce the phenotype and functional changes that occur with disease progression in an ex vivo human model.
methodsSpecimens of normal human fundus were maintained in culture in the presence of serum from patients with achalasia, gastro-oesophageal reflux disease (GORD), or healthy subjects (controls). Immunohistochemical detection of choline acetyltransferase (ChAT), neurone specific enolase (NSE), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), and substance P was carried out in whole mounts of gastric fundus myenteric plexus. In addition, the effects of achalasia serum on electrical field stimulation (EFS) induced contractions were measured in circular muscle preparations.
resultsSerum from achalasia patients did not affect the number of myenteric neurones. Tissues incubated with serum from achalasia patients showed a decrease in the proportion of NOS (-26% of NSE positive neurones; p=0.016) and VIP (-54%; p=0.09) neurones, and a concomitant increase in ChAT neurones (+16%; p<0.001) compared with controls. In contrast, GORD serum did not modify the phenotype of myenteric neurones. Area under the curve of EFS induced relaxations (abolished by N-nitro-L-arginine methyl ester) was significantly decreased following incubation with serum from achalasia patients compared with controls (-7.6 (2.6) v -14.5 (5.0); p=0.036).
conclusionsSerum from achalasia patients can induce phenotypic and functional changes which reproduce the characteristics of the disease. Further identification of putative seric factors and mechanisms involved could lead to the development of novel diagnostic and/or therapeutic strategies in achalasia.
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