ArticlePLoS genetics2005
Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila.
Article in PLoS genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 160 citations in OpenAlex.
- Cross-species incompatibilities offer new insights into the functional consequences of satellite DNA evolution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- An interaction between HP1 and the Chromosomal Passenger Complex Initiates Acentrosomal Spindle Assembly inbioRxiv : the preprint server for biology · 2026Article
- Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway.The EMBO journal · 2026Article
- Binding of heterochromatin protein Rhino to a subset of piRNA clusters depends on a combination of two histone marks.Nature structural & molecular biology · 2025Article
- Article
- Characterization of the Swi6/HP1 binding motif in its partner protein reveals the basis for the functional divergence of the HP1 family proteins in fission yeast.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Prevalent Fast Evolution of Genes Involved in Heterochromatin Functions.Molecular biology and evolution · 2024Article
- Evolutionary adaptation of an HP1-protein chromodomain integrates chromatin and DNA sequence signals.eLife · 2024Article
- Rapid evolution of promoters from germline-specifically expressed genes including transposon silencing factors.BMC genomics · 2024Article
- Molecular Insights into Female Hybrid Sterility in Interspecific Crosses betweenInternational journal of molecular sciences · 2024Article
- Off-target piRNA gene silencing in Drosophila melanogaster rescued by a transposable element insertion.PLoS genetics · 2023Article
- Pathogenic tau-induced transposable element-derived dsRNA drives neuroinflammation.Science advances · 2023Article
- The chromatin factor ROW cooperates with BEAF-32 in regulating long-range inducible genes.EMBO reports · 2022Article
- TheeLife · 2022Article
- Article
- Functions of HP1 proteins in transcriptional regulation.Epigenetics & chromatin · 2022Review
- Functional Diversification of Chromatin on Rapid Evolutionary Timescales.Annual review of genetics · 2021Review
- P-elements strengthen reproductive isolation within the Drosophila simulans species complex.Evolution; international journal of organic evolution · 2021Article
- The Paramount Role ofInsects · 2021Review
- RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility.PLoS genetics · 2021Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heterochromatin comprises a significant component of many eukaryotic genomes. In comparison to euchromatin, heterochromatin is gene poor, transposon rich, and late replicating. It serves many important biological roles, from gene silencing to accurate chromosome segregation, yet little is known about the evolutionary constraints that shape heterochromatin. A complementary approach to the traditional one of directly studying heterochromatic DNA sequence is to study the evolution of proteins that bind and define heterochromatin. One of the best markers for heterochromatin is the heterochromatin protein 1 (HP1), which is an essential, nonhistone chromosomal protein. Here we investigate the molecular evolution of five HP1 paralogs present in Drosophila melanogaster. Three of these paralogs have ubiquitous expression patterns in adult Drosophila tissues, whereas HP1D/rhino and HP1E are expressed predominantly in ovaries and testes respectively. The HP1 paralogs also have distinct localization preferences in Drosophila cells. Thus, Rhino localizes to the heterochromatic compartment in Drosophila tissue culture cells, but in a pattern distinct from HP1A and lysine-9 dimethylated H3. Using molecular evolution and population genetic analyses, we find that rhino has been subject to positive selection in all three domains of the protein: the N-terminal chromo domain, the C-terminal chromo-shadow domain, and the hinge region that connects these two modules. Maximum likelihood analysis of rhino sequences from 20 species of Drosophila reveals that a small number of residues of the chromo and shadow domains have been subject to repeated positive selection. The rapid and positive selection of rhino is highly unusual for a gene encoding a chromosomal protein and suggests that rhino is involved in a genetic conflict that affects the germline, belying the notion that heterochromatin is simply a passive recipient of "junk DNA" in eukaryotic genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.