ArticlePLoS genetics2005
Using microarrays to facilitate positional cloning: identification of tomosyn as an inhibitor of neurosecretion.
Article in PLoS genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- TOM-1/tomosyn acts with the UNC-6/netrin receptor UNC-5 to inhibit growth cone protrusion in Caenorhabditis elegans.Development (Cambridge, England) · 2023Article
- The Role of Tomosyn in the Regulation of Neurotransmitter Release.Advances in neurobiology · 2023Article
- A Hyperactive Form of unc-13 Enhances CaCell reports · 2019Article
- The presynaptic machinery at the synapse of C. elegans.Invertebrate neuroscience : IN · 2018Review
- UNC-18 and Tomosyn Antagonistically Control Synaptic Vesicle Priming Downstream of UNC-13 inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Linking kindling to increased glutamate release in the dentate gyrus of the hippocampus through the STXBP5/tomosyn-1 gene.Brain and behavior · 2017Article
- A Behavioral Survey of the Effects of Kavalactones onJournal of experimental neuroscience · 2017Article
- Distinct Mechanisms Underlie Quiescence during Two Caenorhabditis elegans Sleep-Like States.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- The N- and C-terminal domains of tomosyn play distinct roles in soluble N-ethylmaleimide-sensitive factor attachment protein receptor binding and fusion regulation.The Journal of biological chemistry · 2014Article
- Article
- Coexpressed D1- and D2-like dopamine receptors antagonistically modulate acetylcholine release in Caenorhabditis elegans.Genetics · 2011Article
- Structural and functional analysis of tomosyn identifies domains important in exocytotic regulation.The Journal of biological chemistry · 2011Article
- In vivo analysis of conserved C. elegans tomosyn domains.PloS one · 2011Article
- Parallel pigment and transcriptomic analysis of four barley albina and xantha mutants reveals the complex network of the chloroplast-dependent metabolism.Plant molecular biology · 2009Article
- Defects in tRNA modification associated with neurological and developmental dysfunctions in Caenorhabditis elegans elongator mutants.PLoS genetics · 2009Article
- Friends and foes in synaptic transmission: the role of tomosyn in vesicle priming.Trends in neurosciences · 2009Review
- Profiling synaptic proteins identifies regulators of insulin secretion and lifespan.PLoS genetics · 2008Article
- Insights from retinitis pigmentosa into the roles of isocitrate dehydrogenases in the Krebs cycle.Nature genetics · 2008Article
- Tomosyn negatively regulates both synaptic transmitter and neuropeptide release at the C. elegans neuromuscular junction.The Journal of physiology · 2007Article
- An evolutionarily conserved presynaptic protein is required for isoflurane sensitivity in Caenorhabditis elegans.Anesthesiology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Forward genetic screens have been used as a powerful strategy to dissect complex biological pathways in many model systems. A significant limitation of this approach has been the time-consuming and costly process of positional cloning and molecular characterization of the mutations isolated in these screens. Here, the authors describe a strategy using microarray hybridizations to facilitate positional cloning. This method relies on the fact that premature stop codons (i.e., nonsense mutations) constitute a frequent class of mutations isolated in screens and that nonsense mutant messenger RNAs are efficiently degraded by the conserved nonsense-mediated decay pathway. They validate this strategy by identifying two previously uncharacterized mutations: (1) tom-1, a mutation found in a forward genetic screen for enhanced acetylcholine secretion in Caenorhabditis elegans, and (2) an apparently spontaneous mutation in the hif-1 transcription factor gene. They further demonstrate the broad applicability of this strategy using other known mutants in C. elegans,Arabidopsis, and mouse. Characterization of tom-1 mutants suggests that TOM-1, the C. elegans ortholog of mammalian tomosyn, functions as an endogenous inhibitor of neurotransmitter secretion. These results also suggest that microarray hybridizations have the potential to significantly reduce the time and effort required for positional cloning.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.