ArticleGenes & development2005
The growing pre-mRNA recruits actin and chromatin-modifying factors to transcriptionally active genes.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Non-Canonical Localization of Cardiac Troponins: Expanding Functions or Causing Pathologies?International journal of molecular sciences · 2024Review
- Transcriptomics analysis of ethanol treatment of maleFrontiers in physiology · 2023Article
- Nuclear Actin Dynamics in Gene Expression, DNA Repair, and Cancer.Results and problems in cell differentiation · 2022Article
- Putative Coiled-Coil Domain-Dependent Autoinhibition and Alternative Splicing Determine SHTN1's Actin-Binding Activity.Journal of molecular biology · 2020Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Coupling of RNA Polymerase II Transcription Elongation with Pre-mRNA Splicing.Journal of molecular biology · 2016Review
- The DBHS proteins SFPQ, NONO and PSPC1: a multipurpose molecular scaffold.Nucleic acids research · 2016Article
- Actin, actin-binding proteins, and actin-related proteins in the nucleus.Histochemistry and cell biology · 2016Review
- β- and γ-Actins in the nucleus of human melanoma A375 cells.Histochemistry and cell biology · 2015Article
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- The potential roles of actin in the nucleus.Cell journal · 2015Review
- Involvement of the SATB1/F-actin complex in chromatin reorganization during active cell death.International journal of molecular medicine · 2014Article
- Mechanisms of nuclear actin in chromatin-remodeling complexes.Trends in cell biology · 2014Review
- Transcriptional regulation and nuclear reprogramming: roles of nuclear actin and actin-binding proteins.Cellular and molecular life sciences : CMLS · 2013Review
- Rrp6 is recruited to transcribed genes and accompanies the spliced mRNA to the nuclear pore.RNA (New York, N.Y.) · 2012Article
- Nuclear actin and lamins in viral infections.Viruses · 2012Review
- Nuclear actin and myosins: life without filaments.Nature cell biology · 2011Review
- G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb).The Journal of biological chemistry · 2011Article
- Article
- Nuclear functions of actin.Cold Spring Harbor perspectives in biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the dipteran Chironomus tentans, actin binds to hrp65, a nuclear protein associated with mRNP complexes. Disruption of the actin-hrp65 interaction in vivo by the competing peptide 65-2CTS reduces transcription drastically, which suggests that the actin-hrp65 interaction is required for transcription. We show that the inhibitory effect of the 65-2CTS peptide on transcription is counteracted by trichostatin A, a drug that inhibits histone deacetylation. We also show that actin and hrp65 are associated in vivo with p2D10, an evolutionarily conserved protein with histone acetyltransferase activity that acts on histone H3. p2D10 is recruited to class II genes in a transcription-dependent manner. We show, using the Balbiani ring genes of C. tentans as a model system, that p2D10 is cotranscriptionally associated with the growing pre-mRNA. We also show that experimental disruption of the actin-hrp65 interaction by the 65-2CTS peptide in vivo results in the release of p2D10 from the transcribed genes, reduced histone H3 acetylation, and a lower level of transcription activity. Furthermore, antibodies against p2D10 inhibit run-on elongation. Our results suggest that actin, hrp65, and p2D10 are parts of a positive feedback mechanism that contributes to maintaining the active transcription state of a gene by recruiting HATs at the RNA level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.