ArticleBiophysical journal2005
Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative Fluorescent Speckle Microscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Rethinking contractility in active cytoskeletal matter.Biophysical journal · 2026Article
- Reconstructing Actin Dynamics of the Leading Edge from Observational Data.bioRxiv : the preprint server for biology · 2026Article
- LamelliQuant: An Automated Analysis Pipeline for Lamellipodia Protrusion Dynamics.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- Modulation of the cytoskeleton for cancer therapy.Frontiers in cell and developmental biology · 2025Article
- Nascent adhesions shorten the period of lamellipodium protrusion through the Brownian ratchet mechanism.Molecular biology of the cell · 2023Article
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Coro1B and Coro1C regulate lamellipodia dynamics and cell motility by tuning branched actin turnover.The Journal of cell biology · 2022Article
- Collective migration during a gap closure in a two-dimensional haptotactic model.Scientific reports · 2021Article
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- A composition-dependent molecular clutch between T cell signaling condensates and actin.eLife · 2019Article
- Regulation of axon growth by myosin II-dependent mechanocatalysis of cofilin activity.The Journal of cell biology · 2019Article
- On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility.Molecular biology of the cell · 2018Article
- Discovery of functional interactions among actin regulators by analysis of image fluctuations in an unperturbed motile cell system.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- MIEN1 drives breast tumor cell migration by regulating cytoskeletal-focal adhesion dynamics.Oncotarget · 2016Article
- ERK reinforces actin polymerization to power persistent edge protrusion during motility.Science signaling · 2015Article
- Intrinsic activity in cells and the brain.Molecular biology of the cell · 2014Article
- Mathematical modeling of the dynamic mechanical behavior of neighboring sarcomeres in actin stress fibers.Cellular and molecular bioengineering · 2014Article
- Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones.Molecular biology of the cell · 2013Article
- Dynamic and reversible surface topography influences cell morphology.Journal of biomedical materials research. Part A · 2013Article
Corrections and comments
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6 authors.
Funding
Abstract
We measured actin turnover in lamellipodia and lamellae of migrating cells, using quantitative Fluorescent Speckle Microscopy. Lamellae disassembled at low rates from the front to the back. However, the dominant feature in their turnover was a spatially random pattern of periodic polymerization and depolymerization moving with the retrograde flow. Power spectra contained frequencies between 0.5 and 1 cycle/min. The spectra remained unchanged when applying Latrunculin A and Jasplakinolide in low doses, except that additional frequencies occurred beyond 1 cycle/min. Whereas Latrunculin did not change the rate of mean disassembly, Jasplakinolide halted it completely, indicating that the steady state and the dynamics of actin turnover are differentially affected by pharmacological agents. Lamellipodia assembled in recurring bursts at the leading edge and disassembled approximately 2.5 microm behind. Events of polymerization correlated spatially and temporally with transient formation of Arp2/3 clusters. In lamellae, Arp2/3 accumulation and polymerization correlated only spatially, suggesting an Arp2/3-independent mechanism for filament nucleation. To acquire these data we had to enhance the resolution of quantitative Fluorescent Speckle Microscopy to the submicron level. Several algorithmic advances in speckle image processing are described enabling the analysis of kinetic and kinematic activities of polymer networks at the level of single speckles.
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