ArticleBiophysical journal2005
Homology modeling identifies C-terminal residues that contribute to the Ca2+ sensitivity of a BKCa channel.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 21 citations in OpenAlex.
- Computational Modeling of Genetic Contributions to Excitability and Neural Coding in Layer V Pyramidal Cells: Applications to Schizophrenia Pathology.Frontiers in computational neuroscience · 2019Article
- Endogenous and Agonist-induced Opening of Mitochondrial Big Versus Small Ca2+-sensitive K+ Channels on Cardiac Cell and Mitochondrial Protection.Journal of cardiovascular pharmacology · 2017Article
- Functional Interactions between BKOxidative medicine and cellular longevity · 2016Article
- Protection against cardiac injury by small Ca(2+)-sensitive K(+) channels identified in guinea pig cardiac inner mitochondrial membrane.Biochimica et biophysica acta · 2013Article
- The contribution of RCK domains to human BK channel allosteric activation.The Journal of biological chemistry · 2012Article
- Metal-driven operation of the human large-conductance voltage- and Ca2+-dependent potassium channel (BK) gating ring apparatus.The Journal of biological chemistry · 2011Article
- Relative motion of transmembrane segments S0 and S4 during voltage sensor activation in the human BK(Ca) channel.The Journal of general physiology · 2010Article
- The RCK1 domain of the human BKCa channel transduces Ca2+ binding into structural rearrangements.The Journal of general physiology · 2010Article
- Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution.Science (New York, N.Y.) · 2010Article
- Nardilysin convertase regulates the function of the maxi-K channel isoform mK44 in human myometrium.American journal of physiology. Cell physiology · 2009Article
- Article
- Regulation of bestrophin Cl channels by calcium: role of the C terminus.The Journal of general physiology · 2008Article
- Ethanol modulates BKCa channels by acting as an adjuvant of calcium.Molecular pharmacology · 2008Article
- The RCK2 domain of the human BKCa channel is a calcium sensor.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Tuning magnesium sensitivity of BK channels by mutations.Biophysical journal · 2006Article
- Statistical limits to the identification of ion channel domains by sequence similarity.The Journal of general physiology · 2006Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Activation of BK(Ca) channels by direct Ca(2+) binding and membrane depolarization occur via independent and additive molecular processes. The "calcium bowl" domain is critically involved in Ca(2+)-dependent gating, and we have hypothesized that a sequence within this domain may resemble an EF hand motif. Using a homology modeling strategy, it was observed that a single Ca(2+) ion may be coordinated by the oxygen-containing side chains of residues within the calcium bowl (i.e., (912)ELVNDTNVQFLD(923)). To examine these predictions directly, alanine-substituted BK(Ca) channel mutants were expressed in HEK 293 cells and the voltage and Ca(2+) dependence of macroscopic currents were examined in inside-out membrane patches. Over the range of 1-10 microM free Ca(2+), single point mutations (i.e., E912A and D923A) produced rightward shifts in the steady-state conductance-voltage relations, whereas the mutants N918A or Q920A had no effect on Ca(2+)-dependent gating. The double mutant E912A/D923A displayed a synergistic shift in Ca(2+)-sensitive gating, as well as altered kinetics of current activation/deactivation. In the presence of 1, 10, and 80 mM cytosolic Mg(2+), this double mutation significantly reduced the Ca(2+)-induced free energy change associated with channel activation. Finally, mutations that altered sensitivity of the holo-channel to Ca(2+) also reduced direct (45)Ca binding to the calcium bowl domain expressed as a bacterial fusion protein. These findings, along with other recent data, are considered in the context of the calcium bowl's high affinity Ca(2+) sensor and the known properties of EF hands.
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