ArticleJournal of molecular evolution2005
In vitro selection of high temperature Zn(2+)-dependent DNAzymes.
Article in Journal of molecular evolution, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- DNA Catalysis: Design, Function, and Optimization.Molecules (Basel, Switzerland) · 2024Review
- Simultaneous FeScience advances · 2023Article
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- Functional Nucleic Acid Nanomaterials: Development, Properties, and Applications.Angewandte Chemie (International ed. in English) · 2021Review
- Nucleic Acid Catalysis under Potential Prebiotic Conditions.Chemistry, an Asian journal · 2020Review
- DNAzymes as Activity-Based Sensors for Metal Ions: Recent Applications, Demonstrated Advantages, Current Challenges, and Future Directions.Accounts of chemical research · 2019Review
- Metal-Dependent DNAzymes for the Quantitative Detection of Metal Ions in Living Cells: Recent Progress, Current Challenges, and Latest Results on FRET Ratiometric Sensors.Inorganic chemistry · 2019Review
- Probe computing model based on small molecular switch.BMC bioinformatics · 2019Article
- Fluorometric determination of zinc(II) by using DNAzyme-modified magnetic microbeads.Mikrochimica acta · 2018Article
- Serendipitous Discovery of a Guanine-rich DNA Molecule with a Highly Stable Structure in Urea.Scientific reports · 2018Article
- Near-Infrared Photothermally Activated DNAzyme-Gold Nanoshells for Imaging Metal Ions in Living Cells.Angewandte Chemie (International ed. in English) · 2017Article
- A rapid and visual turn-off sensor for detecting copper (II) ion based on DNAzyme coupled with HCR-based HRP concatemers.Scientific reports · 2017Article
- Theranostic DNAzymes.Theranostics · 2017Review
- Identification of the Same Na(+)-Specific DNAzyme Motif from Two In Vitro Selections Under Different Conditions.Journal of molecular evolution · 2015Article
- DNA Catalysis: The Chemical Repertoire of DNAzymes.Molecules (Basel, Switzerland) · 2015Review
- Photocaged DNAzymes as a general method for sensing metal ions in living cells.Angewandte Chemie (International ed. in English) · 2014Article
- Functional nucleic acid sensors.Chemical reviews · 2009Review
- A self-cleaving DNA enzyme modified with amines, guanidines and imidazoles operates independently of divalent metal cations (M2+).Nucleic acids research · 2009Article
- A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Characterization of long RNA-cleaving deoxyribozymes with short catalytic cores: the effect of excess sequence elements on the outcome of in vitro selection.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro selection of Zn(2+)-dependent RNA-cleaving DNAzymes with activity at 90 degrees C has yielded a diverse spool of selected sequences. The RNA cleavage efficiency was found in all cases to be specific for Zn(2+) over Pb(2+), Ca(2+), Cd(2+), Co(2+), Hg(2+), and Mg(2+). The Zn(2+)-dependent activity assay of the most active sequence showed that the DNAzyme possesses an apparent Zn(2+)-binding dissociation constant of 234 muM and that its activity increases with increasing temperatures from 50-90 degrees C. A fit of the Arrhenius plot data gave E(a) = 15.3 kcal mol(-1). Surprisingly, the selected Zn(2+)-dependent DNAzymes showed only a modest (approximately 3-fold) activity enhancement over the background rate of cleavage of random sequences containing a single embedded ribonucleotide within an otherwise DNA oligonucleotide. The result is attributable to the ability of DNA to sustain cleavage activity at high temperature with minimal secondary structure when Zn(2+) is present. Since this effect is highly specific for Zn(2+), this metal ion may play a special role in molecular evolution of nucleic acids at high temperature.
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