ArticleMolecular biology of the cell2005
Control of Golgi morphology and function by Sed5 t-SNARE phosphorylation.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Retro-2 alters Golgi structure.Scientific reports · 2022Article
- Export Control: Post-transcriptional Regulation of the COPII Trafficking Pathway.Frontiers in cell and developmental biology · 2020Review
- Review
- The Roles of the SNARE Protein Sed5 in Autophagy in Saccharomyces cerevisiae.Molecules and cells · 2017Article
- Potent Inhibition of Human Cytomegalovirus by Modulation of Cellular SNARE Syntaxin 5.Journal of virology · 2017Article
- Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection.Molecular & cellular proteomics : MCP · 2015Article
- Remodeling of secretory compartments creates CUPS during nutrient starvation.The Journal of cell biology · 2014Article
- Get3 is a holdase chaperone and moves to deposition sites for aggregated proteins when membrane targeting is blocked.Journal of cell science · 2013Article
- Article
- Molecular basis for recognition of dilysine trafficking motifs by COPI.Developmental cell · 2012Article
- The yeast Batten disease orthologue Btn1 controls endosome-Golgi retrograde transport via SNARE assembly.The Journal of cell biology · 2011Article
- Golgi biogenesis.Cold Spring Harbor perspectives in biology · 2011Review
- The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.Traffic (Copenhagen, Denmark) · 2010Review
- Bat3 promotes the membrane integration of tail-anchored proteins.Journal of cell science · 2010Article
- Blocking ER export of the Golgi SNARE SYP31 affects plant growth.Plant signaling & behavior · 2009Article
- Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase.The Journal of cell biology · 2008Article
- Article
- Protein phosphatase type 1 directs chitin synthesis at the bud neck in Saccharomyces cerevisiae.Molecular biology of the cell · 2008Article
- A Golgi fragmentation pathway in neurodegeneration.Neurobiology of disease · 2008Article
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we demonstrated that the phosphorylation of t-SNAREs by protein kinase A (PKA) affects their ability to participate in SNARE complexes and to confer endocytosis and exocytosis in yeast. Here, we show that the presumed phosphorylation of a conserved membrane-proximal PKA consensus site (serine-317) in the Sed5 t-SNARE regulates endoplasmic reticulum (ER)-Golgi transport, as well as Golgi morphology. Sed5 is a phosphoprotein, and both alanine and aspartate substitutions in serine-317 directly affect intracellular protein trafficking. The aspartate substitution results in elaboration of the ER, defects in Golgi-ER retrograde transport, an accumulation of small transport vesicles, and the inhibition of growth of most cell types. In contrast, the alanine substitution has no deleterious effects upon transport and growth, but results in ordering of the Golgi into a structure reminiscent of mammalian apparatus. This structure seems to require the recycling of Sed5, because it was found not to occur in sec21-2 cells that are defective in retrograde transport. Thus, a cycle of Sed5 phosphorylation and dephosphorylation is required for normal t-SNARE function and may choreograph Golgi ordering and dispersal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.