ArticleMolecular biology of the cell2005
Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- An activating mutation in AGEF-1, a putative Arf GEF, causes yolk extrusion frombioRxiv : the preprint server for biology · 2026Article
- An activating mutation in AGEF-1, a putative Arf GEF, causes yolk extrusion frommicroPublication biology · 2026Article
- Visualization of Protein Sorting at theFrontiers in cell and developmental biology · 2019Article
- In vivo monitoring of the recruitment and activation of AP-1 by Arf1.Scientific reports · 2017Article
- ArfGAP1 function in COPI mediated membrane traffic: currently debated models and comparison to other coat-binding ArfGAPs.Histology and histopathology · 2012Review
- Novel N-benzoyl-2-hydroxybenzamide disrupts unique parasite secretory pathway.Antimicrobial agents and chemotherapy · 2012Article
- Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor.Plant physiology · 2010Article
- Arf GAP2 is positively regulated by coatomer and cargo.Cellular signalling · 2009Article
- Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.Archives of biochemistry and biophysics · 2008Article
- Arf family GTP loading is activated by, and generates, positive membrane curvature.The Biochemical journal · 2008Article
- Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.Molecular biology of the cell · 2007Article
- Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Proteomic analysis of adaptor protein 1A coats selectively assembled on liposomes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- COPI-mediated transport.The Journal of membrane biology · 2006Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of AP-1/clathrin coat formation was analyzed using purified adaptor proteins and synthetic liposomes presenting tyrosine sorting signals. AP-1 adaptors recruited in the presence of Arf1.GTP and sorting signals were found to oligomerize to high-molecular-weight complexes even in the absence of clathrin. The appendage domains of the AP-1 adaptins were not required for oligomerization. On GTP hydrolysis induced by the GTPase-activating protein ArfGAP1, the complexes were disassembled and AP-1 dissociated from the membrane. AP-1 stimulated ArfGAP1 activity, suggesting a role of AP-1 in the regulation of the Arf1 "GTPase timer." In the presence of cytosol, AP-1 could be recruited to liposomes without sorting signals, consistent with the existence of docking factors in the cytosol. Under these conditions, however, AP-1 remained monomeric, and recruitment in the presence of GTP was short-lived. Sorting signals allowed stable recruitment and oligomerization also in the presence of cytosol. These results suggest a mechanism whereby initial assembly of AP-1 with Arf1.GTP and ArfGAP1 on the membrane stimulates Arf1 GTPase activity, whereas interaction with cargo induces oligomerization and reduces the rate of GTP hydrolysis, thus contributing to efficient cargo sorting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.