ArticleProceedings of the National Academy of Sciences of the United States of America2005
HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 83 citations in OpenAlex.
- HOX genes in stem cells: Maintaining cellular identity and regulation of differentiation.Frontiers in cell and developmental biology · 2022Review
- HOXB4 Promotes Hemogenic Endothelium Formation without Perturbing Endothelial Cell Development.Stem cell reports · 2018Article
- HOXB4 IncreasesTransfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2017Article
- In Vitro Generation of Vascular Wall-Resident Multipotent Stem Cells of Mesenchymal Nature from Murine Induced Pluripotent Stem Cells.Stem cell reports · 2017Article
- Embryonic stem cell-derived haematopoietic progenitor cells down-regulate the CD3 ξ chain on T cells, abrogating alloreactive T cells.Immunology · 2014Article
- Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- USF1 and hSET1A mediated epigenetic modifications regulate lineage differentiation and HoxB4 transcription.PLoS genetics · 2013Article
- Article
- Erythropoiesis: development and differentiation.Cold Spring Harbor perspectives in medicine · 2013Review
- Reprogrammed cells for disease modeling and regenerative medicine.Annual review of medicine · 2013Review
- Serum- and stromal cell-free hypoxic generation of embryonic stem cell-derived hematopoietic cells in vitro, capable of multilineage repopulation of immunocompetent mice.Stem cells translational medicine · 2012Article
- Dynamic HoxB4-regulatory network during embryonic stem cell differentiation to hematopoietic cells.Blood · 2012Article
- Mapping mouse hemangioblast maturation from headfold stages.Developmental biology · 2012Article
- Embryonic stem cell-derived T cells induce lethal graft-versus-host disease and reject allogenic skin grafts upon thymic selection.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Article
- The HOXB4 homeoprotein promotes the ex vivo enrichment of functional human embryonic stem cell-derived NK cells.PloS one · 2012Article
- Immunity of embryonic stem cell-derived hematopoietic progenitor cells.Seminars in immunopathology · 2011Review
- Article
- Experimental limitations using reprogrammed cells for hematopoietic differentiation.Journal of biomedicine & biotechnology · 2011Review
- Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic manipulation of hematopoietic stem and progenitor cells is an important tool for experimental and clinical applied hematology. However, techniques that allow for gene targeting, subsequent in vitro selection, and expansion of genetically defined clones are available only for ES cells. Such molecularly defined and, hence, "safe" clones would be highly desirable for somatic gene therapy. Here, we demonstrate that in vitro differentiated ES cells completely recapitulate the growth and differentiation properties of adult bone marrow cells, in vitro and in vivo, when ectopically expressing HOXB4. Myeloid development was enforced and (T) lymphoid development suppressed over a wide range of expression levels, whereas only high expression levels of the transcription factor were detrimental for erythroid development. This indicates a close association between the amounts of ectopic HOXB4 present within a progenitor cell and and the decision to self renew or differentiate. Because HOXB4 mediates similar fates of ES-derived and bone marrow hematopoietic stem cells, the primitive embryonic cells can be considered a promising alternative for investigating hematopoietic reconstitution, in vivo, based on well defined clones. Provided that HOXB4 levels are kept within a certain therapeutic window, ES cells also carry the potential of efficient and safe somatic gene therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.