Evidence map›Paper›PMID 16092970›Full record

ArticleRetrovirology2005

Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera.

Wu Ou, Jonathan Silver

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Retrovirology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Wu OuLaboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Bethesda, MD 20892, USA. wou@niaid.nih.gov
Jonathan Silver
National Institute of Allergy and Infectious Diseases · US

Funding

Retrovirus-cell InteractionsZ01AI000781 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI SILVER, JONATHAN · 1997 to 2007
$153k
6 · The paper itself

Abstract

backgroundThe N-heptad repeat region of the HIV-1 Transmembrane Envelope protein is a trimerization domain that forms part of a "six helix bundle" crucial to Envelope-mediated membrane fusion. N-heptad repeat peptides have been used as extracellular reagents to inhibit virus fusion.

resultsWhen expressed intracellularly with wild-type HIV-1 Envelope protein, the N-heptad repeat domain efficiently hetero-oligomerized with Envelope and trapped it in the endoplasmic reticulum or early Golgi, as indicated by lack of transport to the cell surface, absent proteolytic processing, and aberrant glycosylation.

conclusionPost-translational processing of HIV Envelope is very sensitive to an agent that binds to the N-heptad repeat during synthesis, suggesting that it might be possible to modify drugs that bind to this region to have transport-blocking properties.

Indexed as

Anti-HIV AgentsBacterial ProteinsBiotinylationEndoplasmic ReticulumGene Products, envHeLa CellsHIV-1HumansLuminescent ProteinsMembrane FusionProtein Processing, Post-TranslationalProtein Structure, SecondaryProtein TransportReceptors, CCR5Recombinant Fusion ProteinsRepetitive Sequences, Amino AcidAnti-HIV AgentsBacterial ProteinsGene Products, envLuminescent ProteinsReceptors, CCR5Recombinant Fusion Proteinsyellow fluorescent protein, Bacteria

Identifiers

PMID16092970
PMCPMC1199619
OpenAlexW2168318730

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.