ArticleNucleic acids research2005
Direct detection of methylation in genomic DNA.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Empirical Comparison and Analysis of Web-Based DNAMolecular therapy. Nucleic acids · 2020Article
- A novel DNA methylation motif identified in Bacillus pumilus BA06 and possible roles in the regulation of gene expression.Applied microbiology and biotechnology · 2020Article
- A Small RNA Transforms the Multidrug Resistance of Pseudomonas aeruginosa to Drug Susceptibility.Molecular therapy. Nucleic acids · 2019Article
- Deciphering bacterial epigenomes using modern sequencing technologies.Nature reviews. Genetics · 2019Review
- N6-Methyladenine DNA modification in Xanthomonas oryzae pv. oryzicola genome.Scientific reports · 2018Article
- Intermolecular 'cross-torque': the N4-cytosine propargyl residue is rotated to the 'CH'-edge as a result of Watson-Crick interaction.Nucleic acids research · 2015Article
- DNA Methylation.EcoSal Plus · 2014Article
- Entering the era of bacterial epigenomics with single molecule real time DNA sequencing.Current opinion in microbiology · 2013Review
- DNA methylation impacts gene expression and ensures hypoxic survival of Mycobacterium tuberculosis.PLoS pathogens · 2013Article
- Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing.Nucleic acids research · 2012Article
- Article
- Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.Journal of clinical microbiology · 2011Article
- YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of methylated sites on bacterial genomic DNA would be a useful tool to study the major roles of DNA methylation in prokaryotes: distinction of self and nonself DNA, direction of post-replicative mismatch repair, control of DNA replication and cell cycle, and regulation of gene expression. Three types of methylated nucleobases are known: N6-methyladenine, 5-methylcytosine and N4-methylcytosine. The aim of this study was to develop a method to detect all three types of DNA methylation in complete genomic DNA. It was previously shown that N6-methyladenine and 5-methylcytosine in plasmid and viral DNA can be detected by intersequence trace comparison of methylated and unmethylated DNA. We extended this method to include N4-methylcytosine detection in both in vitro and in vivo methylated DNA. Furthermore, application of intersequence trace comparison was extended to bacterial genomic DNA. Finally, we present evidence that intrasequence comparison suffices to detect methylated sites in genomic DNA. In conclusion, we present a method to detect all three natural types of DNA methylation in bacterial genomic DNA. This provides the possibility to define the complete methylome of any prokaryote.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.