ArticleAmerican journal of physiology. Renal physiology2006
UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct.
Article in American journal of physiology. Renal physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Genes and Evolution of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Transgenic Restoration of Urea Transporter A1 Confers Maximal Urinary Concentration in the Absence of Urea Transporter A3.Journal of the American Society of Nephrology : JASN · 2016Article
- Water restriction increases renal inner medullary manganese superoxide dismutase (MnSOD).American journal of physiology. Renal physiology · 2012Article
- The emerging physiological roles of the SLC14A family of urea transporters.British journal of pharmacology · 2011Review
- Role of collecting duct urea transporters in the kidney--insights from mouse models.The Journal of membrane biology · 2006Review
- Genomic organization of the mammalian SLC14a2 urea transporter genes.The Journal of membrane biology · 2006Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The urea transporters, UT-A1 and UT-A3, two members of the UT-A gene family, are localized to the terminal portion of the inner medullary collecting duct (IMCD). In this manuscript, we demonstrate that 4.2 kb of the 5'-flanking region of the UT-A gene (UT-Aalpha promoter) is sufficient to drive the IMCD-specific expression of a heterologous reporter gene, beta-galactosidase (beta-Gal), in transgenic mice. RT-PCR, immunoblotting, and immunohistochemistry demonstrate that within the kidney, transgene expression is confined to the terminal portion of the IMCD. Colocalization studies with aquaporin-2 show that expression is localized to the principal cells of the IMCD2 and IMCD3 regions. Utilizing beta-Gal activity assays, we further show that within the kidney, the beta-Gal transgene can be regulated by both water restriction and glucocorticoids, similar to the regulation of the endogenous UT-A gene. These results demonstrate that 4.2 kb of the UT-Aalpha promoter is sufficient to drive expression of a heterologous reporter gene in a tissue-specific and cell-specific fashion in transgenic mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.