ArticleProceedings of the National Academy of Sciences of the United States of America1992
Differentiation of renal beta-intercalated cells to alpha-intercalated and principal cells in culture.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 95 citations in OpenAlex.
- Molecular mechanism of p-Coumaric acid in the regulation of AQP2 function through the activation of calcium-sensing receptor signaling.Scientific reports · 2026Article
- Renal Intercalated Cells: Alien Cells Inside Us?Biology · 2025Review
- A Possible Link between Cell Plasticity and Renin Expression in the Collecting Duct: A Narrative Review.International journal of molecular sciences · 2024Review
- The transcription factor Foxi1 promotes expression of V-ATPase and Gpr116 in M-1 cells.American journal of physiology. Renal physiology · 2023Article
- Human ureteric bud organoids recapitulate branching morphogenesis and differentiate into functional collecting duct cell types.Nature biotechnology · 2023Article
- Mapping the human kidney using single-cell genomics.Nature reviews. Nephrology · 2022Review
- Connecting tubules develop from the tip of the ureteric bud in the human kidney.Histochemistry and cell biology · 2021Article
- Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis.International journal of molecular sciences · 2021Article
- Effect of luminal flow on doming of mpkCCD cells in a 3D perfusable kidney cortical collecting duct model.American journal of physiology. Cell physiology · 2020Article
- Acidosis induces antimicrobial peptide expression and resistance to uropathogenicAmerican journal of physiology. Renal physiology · 2020Article
- Cell-specific qRT-PCR of renal epithelial cells reveals a novel innate immune signature in murine collecting duct.American journal of physiology. Renal physiology · 2018Article
- Chronic lithium treatment induces novel patterns of pendrin localization and expression.American journal of physiology. Renal physiology · 2018Article
- Precision Medicine for Acute Kidney Injury (AKI): Redefining AKI by Agnostic Kidney Tissue Interrogation and Genetics.Seminars in nephrology · 2018Review
- Transcription factoreLife · 2017Article
- Elf5 is a principal cell lineage specific transcription factor in the kidney that contributes to Aqp2 and Avpr2 gene expression.Developmental biology · 2017Article
- Aquaporin 2-labeled cells differentiate to intercalated cells in response to potassium depletion.Histochemistry and cell biology · 2016Article
- Development of the Mammalian Kidney.Current topics in developmental biology · 2016Review
- Integrated compensatory network is activated in the absence of NCC phosphorylation.The Journal of clinical investigation · 2015Article
- Adam10 mediates the choice between principal cells and intercalated cells in the kidney.Journal of the American Society of Nephrology : JASN · 2015Article
- Lack of an effect of collecting duct-specific deletion of adenylyl cyclase 3 on renal Na+ and water excretion or arterial pressure.American journal of physiology. Renal physiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The renal collecting duct is a heterogenous epithelium consisting of intercalated cells (ICC) and principal cells (PC). The origin of this cellular heterogeneity is not clear. To test the hypothesis that the two cell types might originate from one another, pure populations of ICC (beta subtype) and PC were isolated by fluorescence-activated cell sorting and grown on permeable supports. After the monolayers reached confluence, the expression of ICC- and PC-specific functions and antigens was monitored. Cultures of sorted beta-ICC, in addition to expressing ICC-specific functions (such as an electrogenic H+ secretion) and antigens, progressively acquired PC functions (amiloride-sensitive Na+ transport and K+ secretion). On day 6, cultures of sorted beta-ICC exhibited a lumen-negative transepithelial potential difference of 83 +/- 4 mV and a short circuit current of 107 +/- 15 microA/cm2 and created a lumen-to-bath K+ concentration ratio of approximately 10. The percentage of cells staining with two PC-specific antibodies was 53% and 65%. On the other hand, cultures of sorted PC failed to acquire ICC-specific functions while maintaining PC characteristics. To rule out preferential proliferation of a few contaminating PC as an explanation of these results, we have generated a continuous collecting duct cell line (M-1) originating from mice transgenic for the early region of simian virus 40. Cell lines cloned from M-1 cells exhibit both PC and ICC functions and show mutually exclusive heterogenous expression of PC and ICC antigens, demonstrating a common origin of the two cell types. These data indicate that while beta-ICC in culture can give rise to both alpha-ICC and PC, PC cannot convert to ICC, which raises the possibility that beta-ICC is the stem cell in the renal collecting duct. Differentiation of ICC to PC may explain the cellular heterogeneity in the cortical collecting duct.
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