ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2005
Effects of spironolactone and RU486 on gene expression and cell proliferation after freshwater transfer in the euryhaline killifish.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 50 citations in OpenAlex.
- Regulation and function of the gill corticotropin-releasing factor system during osmoregulatory disturbances in Atlantic salmon.The Journal of experimental biology · 2025Article
- Endocrine control of gill ionocyte function in euryhaline fishes.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- Evolutionarily conserved mechanisms regulating stress-induced neutrophil redistribution in fish.Frontiers in immunology · 2024Article
- 11-Deoxycortisol controls hydromineral balance in the most basal osmoregulating vertebrate, sea lamprey (Petromyzon marinus).Scientific reports · 2020Article
- Metabolomics for informing adverse outcome pathways: Androgen receptor activation and the pharmaceutical spironolactone.Aquatic toxicology (Amsterdam, Netherlands) · 2017Article
- Environmental and cortisol-mediated control of Ca(2+) uptake in tilapia (Oreochromis mossambicus).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Estrogen and glucocorticoid receptor agonists and antagonists in oocytes modulate the pattern of expression of genes that encode nuclear receptor proteins in very early stage rainbow trout (Oncorhynchus mykiss) embryos.Fish physiology and biochemistry · 2015Article
- Article
- Glucocorticoid receptor blockade inhibits brain cell addition and aggressive signaling in electric fish, Apteronotus leptorhynchus.Hormones and behavior · 2011Article
- Fundulus as the premier teleost model in environmental biology: opportunities for new insights using genomics.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2007Article
- Metabolic and ionoregulatory responses of the Amazonian cichlid, Astronotus ocellatus, to severe hypoxia.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have explored the possible mechanisms by which mineralocorticoid (MR) and glucocorticoid (GR) receptors regulate the response to freshwater transfer in the gills of the euryhaline killifish Fundulus heteroclitus. Killifish were implanted with RU486 (GR antagonist) or spironolactone (MR antagonist) at doses of 0.1-1.0 mg g(-1), and subsequently transferred from 10 per thousand brackish water to freshwater. Compared to brackish water sham fish, mRNA expression of CFTR and NKCC1 decreased in the gills of sham fish transferred to freshwater, whereas Na(+), K(+)-ATPase alpha(1a) mRNA expression and alpha protein abundance, as well as cell proliferation (detected using BrdU) increased. Spironolactone inhibited the normal increase in cell proliferation and Na(+), K(+)-ATPase expression after freshwater transfer. RU486 increased plasma cortisol levels and may have slightly inhibited Na(+), K(+)-ATPase activity, but did not change alpha(1a ) expression. RU486 had no effect on cell proliferation in the non-lamellar region of the gills, but increased proliferation in the lamellar region. Neither antagonist inhibited the suppression of CFTR or NKCC1 expression after freshwater transfer. Glucocorticoid receptor expression was reduced in all sham and antagonist treatments compared to untreated controls, but no other consistent differences were observed. The effects of spironolactone suggest that MR is important for regulating ion transport in killifish gills after freshwater transfer.
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