ArticleEukaryotic cell2005
Cryptococcus neoformans gene expression during murine macrophage infection.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
117 citing papers in PubMed, 186 citations in OpenAlex.
- Mammalian-evolved strains of the fungal pathogen Cryptococcus neoformans show adaptation via heritable transcript level differences specific to mouse MHC haplotype.BMC genomics · 2026Article
- Fluorescent organelle markers in Cryptococcus neoformans: a versatile toolkit for live-cell subcellular localization.Genetics · 2026Article
- Genomic and physiological signatures of adaptation in pathogenic fungi.Nature communications · 2026Article
- Thermotolerant insect-associated selective pressure may drive increased lipid metabolic plasticity and emerging pathogenic potential in yeasts.Frontiers in microbiology · 2026Article
- Extracellular Vesicles From Fungal Infection in Humans: A Key Player in Immunological Responses.Journal of extracellular biology · 2025Article
- Peroxisomes regulate virulence and cell density sensing inbioRxiv : the preprint server for biology · 2025Article
- An Introduction to the Influence of Nutritional Factors on the Pathogenesis of Opportunist Fungal Pathogens in Humans.Pathogens (Basel, Switzerland) · 2025Review
- Cryptococcal nutrient acquisition and pathogenesis: dining on the host.Microbiology and molecular biology reviews : MMBR · 2025Review
- Article
- A fungal protein organizes both glycogen and cell wall glucans.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides fromJournal of fungi (Basel, Switzerland) · 2024Article
- A Limited Number of Amino Acid Permeases Are Crucial forInternational journal of microbiology · 2024Review
- Measuring Stress Phenotypes in Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Transcription Factor StuA Regulates the Glyoxylate Cycle in the DermatophyteInternational journal of molecular sciences · 2023Article
- The Response ofJournal of fungi (Basel, Switzerland) · 2023Article
- Amoeba predation of Cryptococcus: A quantitative and population genomic evaluation of the accidental pathogen hypothesis.PLoS pathogens · 2023Article
- Importance of Clinical Isolates inJournal of fungi (Basel, Switzerland) · 2023Review
- Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions.Scientific reports · 2022Article
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
- Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The fungal pathogen Cryptococcus neoformans survives phagocytosis by macrophages and proliferates within, ultimately establishing latent infection as a facultative intracellular pathogen that can escape macrophage control to cause disseminated disease. This process is hypothesized to be important for C. neoformans pathogenesis; however, it is poorly understood how C. neoformans adapts to and overcomes the hostile intracellular environment of the macrophage. Using DNA microarray technology, we have investigated the transcriptional response of C. neoformans to phagocytosis by murine macrophages. The expression profiles of several genes were verified using quantitative reverse transcription-PCR and a green fluorescent protein reporter strain. Multiple membrane transporters for hexoses, amino acids, and iron were up-regulated, as well as genes involved in responses to oxidative stress. Genes involved in autophagy, peroxisome function, and lipid metabolism were also induced. Interestingly, almost the entire mating type locus displayed increased expression 24 h after internalization, suggesting an intrinsic connection between infection and the MAT locus. Genes in the Gpa1-cyclic AMP-protein kinase A pathway were also up-regulated. Both gpa1 and pka1 mutants were found to be compromised in macrophage infection, confirming the important role of this virulence pathway. A large proportion of the repressed genes are involved in ribosome-related functions, rRNA processing, and translation initiation/elongation, implicating a reduction in translation as a central response to phagocytosis. In summary, this gene expression profile allows us to interpret the adaptation of C. neoformans to the intracellular infection process and informs the search for genes encoding novel virulence attributes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.