ArticleNucleic acids research2005
Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Structure, function, and applications of two novel phage recombinases from extreme environments.Nucleic acids research · 2026Article
- A flexible, cost- and time-saving method for in-house laboratory production of DNA markers.Biotechnology letters · 2025Article
- Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.Nucleic acids research · 2023Article
- Real-time fluorescence and colorimetric identification of bulbus fritillariae using recombinase assisted loop-mediated isothermal DNA amplification (RALA).Frontiers in plant science · 2022Article
- An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.PloS one · 2022Article
- Nonfilament-forming RecA dimer catalyzes homologous joint formation.Nucleic acids research · 2018Article
- New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.PLoS genetics · 2018Article
- A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of ClJournal of neurophysiology · 2017Article
- Covalent modification of primers improves PCR amplification specificity and yield.Biology methods & protocols · 2017Article
- Application of a Euryarchaeota-Specific Helicase from Thermococcus kodakarensis for Noise Reduction in PCR.Applied and environmental microbiology · 2016Article
- Highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances specificity of simplex and multiplex PCR assays.Journal of applied genetics · 2016Article
- CdTe quantum dots enhance feasibility of EvaGreen-based real-time PCR with decent amplification fidelity.Molecular biotechnology · 2013Article
- Improved loop-mediated isothermal amplification for HLA-DRB1 genotyping using RecA and a restriction enzyme for enhanced amplification specificity.Immunogenetics · 2013Article
- Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR).International journal of molecular sciences · 2013Article
- Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR.Journal of nucleic acids · 2013Article
- Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments.Plant methods · 2012Article
- A network of conserved co-occurring motifs for the regulation of alternative splicing.Nucleic acids research · 2010Article
- Direct multiplex sequencing (DMPS)--a novel method for targeted high-throughput sequencing of ancient and highly degraded DNA.Genome research · 2009Article
- 3'-Protected 2'-deoxynucleoside 5'-triphosphates as a tool for heat-triggered activation of polymerase chain reaction.Analytical chemistry · 2009Article
- Chemically modified primers for improved multiplex polymerase chain reaction.Analytical biochemistry · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, is due to its promotion of precise priming in PCR (i.e. priming at sites where the primer sequence is completely complementary to that of the target sequence). In addition, the RecA protein substantially reduces the primer concentration required for PCR. These experimental results have led to the realization of multiplex PCR, which involves PCR for multiple sites in the same reaction mixture. We were able to successfully perform multiplex PCR with over a dozen reactions without affecting the amplification pattern of the PCR products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.