ArticleThe Journal of experimental medicine2005
Characterization of the early stages of thymic NKT cell development.
Article in The Journal of experimental medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
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Who cites it
152 citing papers in PubMed.
- Beyond subsets: single cell transcriptomics reveals multidimensional regulation of iNKT cells cross tissues and species.Frontiers in immunology · 2026Review
- Single-cell transcriptomic profiling reveals diversity in human iNKT cells across hematologic tissues.Cell reports · 2025Article
- Evidence for a RIPK1-independent survival mechanism for CASPASE-8 in αβ T cells.Discovery immunology · 2025Article
- NKT Cells and Other Innate T Cells: The Immune Cells That Do Not Follow the Rules.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Eed-dependent histone modification orchestrates the iNKT cell developmental program alleviating liver injury.Frontiers in immunology · 2024Article
- invariant Natural Killer T cell therapy as a novel therapeutic approach in hematological malignancies.Frontiers in transplantation · 2024Review
- Intrinsic factors and CD1d1 but not CD1d2 expression levels control invariant natural killer T cell subset differentiation.Nature communications · 2023Article
- TGF-β Promotes the Postselection Thymic Development and Peripheral Function of IFN-γ-Producing Invariant NKT cells.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Markers and makers of NKT17 cells.Experimental & molecular medicine · 2023Review
- Impaired thymic iNKT cell differentiation at early precursor stage in murine haploidentical bone marrow transplantation with GvHD.Frontiers in immunology · 2023Article
- Antigen-presenting T cells provide critical B7 co-stimulation for thymic iNKT cell development via CD28-dependent trogocytosis.Cell reports · 2022Article
- Thymic epithelial cells require lipid kinase Vps34 for CD4 but not CD8 T cell selection.The Journal of experimental medicine · 2022Article
- METTL14-dependent mCell reports · 2022Article
- Article
- Current insights in mouse iNKT and MAIT cell development using single cell transcriptomics data.Seminars in immunology · 2022Review
- In vivo availability of the cytokine IL-7 constrains the survival and homeostasis of peripheral iNKT cells.Cell reports · 2022Article
- Protein abundance of the cytokine receptor γc controls the thymic generation of innate-like T cells.Cellular and molecular life sciences : CMLS · 2021Article
- Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells.Scientific reports · 2021Article
- The impact of stress on the transcriptomic signature of iNKT1 cells.Biochemistry and biophysics reports · 2021Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).European journal of immunology · 2021Review
92 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Upon reaching the mature heat stable antigen (HSA)low thymic developmental stage, CD1d-restricted Valpha14-Jalpha18 thymocytes undergo a well-characterized sequence of expansion and differentiation steps that lead to the peripheral interleukin-4/interferon-gamma-producing NKT phenotype. However, their more immature HSAhigh precursors have remained elusive, and it has been difficult to determine unambiguously whether NKT cells originate from a CD4+ CD8+ double-positive (DP) stage, and when the CD4+ and CD4- CD8- double-negative (DN) NKT subsets are formed. Here, we have used a CD1d tetramer-based enrichment strategy to physically identify HSAhigh precursors in thymuses of newborn mice, including an elusive DPlow stage and a CD4+ stage, which were present at a frequency of approximately 10(-6). These HSAhigh DP and CD4+ stages appeared to be nondividing, and already exhibited the same Vbeta8 bias that characterizes mature NKT cells. This implied that the massive expansion of NKT cells is separated temporally from positive selection, but faithfully amplifies the selected TCR repertoire. Furthermore, we found that, unlike the DN gammadelta T cells, the DN NKT cells did not originate from a pTalpha-independent pathway bypassing the DP stage, but instead were produced during a short window of time from the conversion of a fraction of HSAlow NK1.1neg CD4 cells. These findings identify the HSAhigh CD4+ stage as a potential branchpoint between NKT and conventional T lineages and between the CD4 and DN NKT sublineages.
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