ArticleGenome biology2005
The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles.
Article in Genome biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Loss of Copine D Leads to Ras Activation inCells · 2026Article
- Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration.Nature communications · 2025Article
- Spontaneous signal generation by an excitable system for cell migration.Frontiers in cell and developmental biology · 2024Review
- Ras inhibitors gate chemoattractant concentration range for chemotaxis through controlling GPCR-mediated adaptation and cell sensitivity.Frontiers in immunology · 2022Review
- Interactome and evolutionary conservation of Dictyostelid small GTPases and their direct regulators.Small GTPases · 2022Article
- Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells.Journal of cell science · 2019Article
- Function of small GTPases in Dictyostelium macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- The novel RacE-binding protein GflB sharpens Ras activity at the leading edge of migrating cells.Molecular biology of the cell · 2016Article
- A Model for Direction Sensing in Dictyostelium discoideum: Ras Activity and Symmetry Breaking Driven by a Gβγ-Mediated, Gα2-Ric8 -- Dependent Signal Transduction Network.PLoS computational biology · 2016Article
- Article
- Cell migration: regulation of cytoskeleton by Rap1 in Dictyostelium discoideum.Journal of microbiology (Seoul, Korea) · 2012Review
- Single and multiple CH (calponin homology) domain containing multidomain proteins in Dictyostelium discoideum: an inventory.Molecular biology reports · 2010Article
- A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration.Developmental cell · 2010Article
- Intramolecular activation mechanism of the Dictyostelium LRRK2 homolog Roco protein GbpC.The Journal of biological chemistry · 2008Article
- PIP3-independent activation of TorC2 and PKB at the cell's leading edge mediates chemotaxis.Current biology : CB · 2008Article
- Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions.The Journal of cell biology · 2008Article
- Filamin repeat segments required for photosensory signalling in Dictyostelium discoideum.BMC cell biology · 2007Article
- Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs.EMBO reports · 2007Article
- High-throughput analysis of spatio-temporal dynamics in Dictyostelium.Genome biology · 2007Article
- Phosphoinositide 3-kinase activity controls the chemoattractant-mediated activation and adaptation of adenylyl cyclase.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDictyostelium discoideum is a eukaryote with a simple lifestyle and a relatively small genome whose sequence has been fully determined. It is widely used for studies on cell signaling, movement and multicellular development. Ras guanine-nucleotide exchange factors (RasGEFs) are the proteins that activate Ras and thus lie near the top of many signaling pathways. They are particularly important for signaling in development and chemotaxis in many organisms, including Dictyostelium.
resultsWe have searched the genome for sequences encoding RasGEFs. Despite its relative simplicity, we find that the Dictyostelium genome encodes at least 25 RasGEFs, with a few other genes encoding only parts of the RasGEF consensus domains. All appear to be expressed at some point in development. The 25 genes include a wide variety of domain structures, most of which have not been seen in other organisms. The LisH domain, which is associated with microtubule binding, is seen particularly frequently; other domains that confer interactions with the cytoskeleton are also common. Disruption of a sample of the novel genes reveals that many have clear phenotypes, including altered morphology and defects in chemotaxis, slug phototaxis and thermotaxis.
conclusionThese results suggest that the unexpectedly large number of RasGEF genes reflects an evolutionary expansion of the range of Ras signaling rather than functional redundancy or the presence of multiple pseudogenes.
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