ArticleClinical and diagnostic laboratory immunology2005
Seroreactivity to and avidity for recombinant antigens in toxoplasmosis.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 114 citations in OpenAlex.
- Investigation of Specific IgG-Secreting Cells in Congenital Toxoplasmosis: The TOXODIAG Study.Journal of clinical laboratory analysis · 2026Article
- The AB Blood Group System Phenotype Does Not Play a Role inPathogens (Basel, Switzerland) · 2025Article
- The Trivalent Recombinant Chimeric Proteins Containing Immunodominant Fragments ofInternational journal of molecular sciences · 2025Article
- Serological diagnosis of toxoplasmosis in pregnancy: comparison between a manual commercial ELISA assay and the automated VIDASEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- IgG Avidity Test as a Tool for Discrimination between Recent and DistantAntibodies (Basel, Switzerland) · 2022Review
- Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens.Parasite (Paris, France) · 2022Article
- Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis.Pathogens (Basel, Switzerland) · 2021Article
- Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans.Journal of clinical microbiology · 2021Article
- Descriptive Comparison of ELISAs for the Detection ofPathogens (Basel, Switzerland) · 2021Review
- Role of Toxoplasma gondii IgG Avidity Testing in Discriminating between Acute and Chronic Toxoplasmosis in Pregnancy.Journal of clinical microbiology · 2020Review
- Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans.Frontiers in cellular and infection microbiology · 2020Review
- Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection ofInfection and drug resistance · 2019Article
- Discovery of new Toxoplasma gondii antigenic proteins using a high throughput protein microarray approach screening sera of murine model infected orally with oocysts and tissue cysts.Parasites & vectors · 2018Article
- Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection.Infection · 2018Review
- Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella.Memorias do Instituto Oswaldo Cruz · 2017Article
- Detection of Toxoplasma gondii in chicken and soil of chicken farms in Nanjing region, China.Infectious diseases of poverty · 2017Article
- Immunoreactivity Analysis of Toxoplasma gondii Recombinant Antigen rSAG3 in Sera from Immunized BALB/c Mice and Toxoplasmosis Patients.Iranian journal of public health · 2016Article
- Diagnosis of toxoplasmosis and typing of Toxoplasma gondii.Parasites & vectors · 2015Review
- Toxoplasma gondii exposure in patients suffering from mental and behavioral disorders due to psychoactive substance use.BMC infectious diseases · 2015Article
- CCp5A Protein from Toxoplasma gondii as a Serological Marker of Oocyst-driven Infections in Humans and Domestic Animals.Frontiers in microbiology · 2015Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve serodiagnostic methods for the diagnosis of acute toxoplasmosis during pregnancy, a new test system has been developed and evaluated based on the use of recombinant antigens. Five recombinant Toxoplasma gondii antigens (ROP1, MAG1, SAG1, GRA7, and GRA8) were cloned in Escherichia coli, purified, and applied directly onto nitrocellulose membranes in a line assay (recomLine Toxoplasma). A panel of 102 sera from 25 pregnant women with supposed recent toxoplasmosis and from two symptomatic children was compared to a panel of 71 sera from individuals with past infection. Both panels were analyzed using a recombinant line assay for immunoglobulin G (IgG), IgM, and IgA antibodies and a reference enzyme-linked immunosorbent assay. Within the IgM-positive samples, antibodies against ROP1 were predominant regardless of the infection state. In IgG analysis a characteristic antibody pattern was found for very recent infections. This pattern changed to a different one during the time course of infection: antibodies against GRA7 and GRA8 were characteristic for very early IgG, whereas antibodies against SAG1 and MAG1 appeared significantly later. These results were further confirmed by determination of the IgG antibody avidity for every single recombinant antigen. In the time course of infection, IgG antibodies against the early recognized antigens matured significantly earlier than those directed against the later antigens did. The IgA patterns did not give reliable information about the infection time points. The data revealed that the recombinant line assay provides valuable information on the actual state of infection, especially during the early infection time points.
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