ArticleClinical and diagnostic laboratory immunology2005
Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Cutaneous Lyme borreliosis: Guideline of the German Dermatology Society.German medical science : GMS e-journal · 2025Guideline
- Cutaneous Lyme borreliosis: Guideline of the German Dermatology Society.German medical science : GMS e-journal · 2017Guideline
- Lyme borreliosis diagnosis: state of the art of improvements and innovations.BMC microbiology · 2023Review
- Large-Scale Sequencing ofGenes · 2022Review
- Quantified Detection of Treponema pallidum DNA by PCR Assays in Urine and Plasma of Syphilis Patients.Microbiology spectrum · 2022Article
- Real time micro-organisms PCR in 104 patients with polymorphic signs and symptoms that may be related to a tick bite.European journal of microbiology & immunology · 2021Article
- Identification of Urine Metabolites as Biomarkers of Early Lyme Disease.Scientific reports · 2018Article
- Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.Analytical methods : advancing methods and applications · 2017Article
- Chronic Lyme disease.Infectious disease clinics of North America · 2015Review
- Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.Journal of biomolecular techniques : JBT · 2014Article
- DNA persistence after treatment of Lyme borreliosis.Folia microbiologica · 2014Article
- Improving urine sample efficacy as a convenient alternative for invasive samples in molecular diagnosis of toxoplasmosis.Iranian journal of parasitology · 2013Article
- Improved culture conditions for the growth and detection of Borrelia from human serum.International journal of medical sciences · 2013Article
- The use of hydrogel microparticles to sequester and concentrate bacterial antigens in a urine test for Lyme disease.Biomaterials · 2011Article
- Novel Diagnosis of Lyme Disease: Potential for CAM Intervention.Evidence-based complementary and alternative medicine : eCAM · 2009Article
- Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many approaches were made in recent years to establish urine PCR as a diagnostic tool for Lyme borreliosis, but results are contradictory. In the present study, a standardized protocol spiking urine from healthy donors with a defined amount of whole Borrelia or Borrelia DNA was established. The development of a nested real-time PCR targeting ospA enabled a highly sensitive and quantitative analysis of these samples. We show the following. (i) Storage of spiked urine samples for up to 6 months at--20 degrees C had no negative effect on spike recovery. (ii) Centrifugation of 10 ml of urine at 40,000 x g for 30 min resulted in a concentration of both spikes, i.e., whole Borrelia and DNA. (iii) The inhibition of DNA spike recovery in 48% (11 of 23 samples) of urine samples tested could be attributed to nuclease activity. This was abrogated by alkalizing the urine or by working with the samples on ice. Despite optimized conditions, analysis of urine samples of 12 patients with erythema migrans, the clinical stage considered to be associated with the highest bacterial load, revealed a positive result in only one sample. All 12 samples were negative by an alternative PCR targeting flagellin. The results of our study support doubts that urine is a suitable material for diagnosis of Lyme borreliosis.
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