ArticleApplied and environmental microbiology2005
Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- A sensitive method for the recovery of Escherichia coli serogroup O55 including Shiga toxin-producing variants for potential use in outbreaks.Journal of applied microbiology · 2019Article
- Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR.BMC microbiology · 2019Article
- Application of lab-on-a-chip multiplex cassette PCR for the detection of enterohemorrhagic Escherichia coli.BMC microbiology · 2019Article
- Variability in CharacterizingMicroorganisms · 2018Article
- Progress in Our Understanding of Wzx Flippase for Translocation of Bacterial Membrane Lipid-Linked Oligosaccharide.Journal of bacteriology · 2018Review
- Molecular Characterization of Enterotoxin-Producing Escherichia coli Collected in 2011-2012, Russia.PloS one · 2015Article
- Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran.Biological research · 2014Article
- Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products.BMC research notes · 2014Article
- Article
- Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran.Gut pathogens · 2013Article
- Article
- Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups.Applied and environmental microbiology · 2012Article
- Association of nucleotide polymorphisms within the O-antigen gene cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with serogroups and genetic subtypes.Applied and environmental microbiology · 2012Article
- Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.Applied and environmental microbiology · 2012Article
- Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk: serogroups, virulence factors, and antibiotic resistance properties.TheScientificWorldJournal · 2012Article
- Molecular characterization of Shiga toxin-producing Escherichia coli isolated from ruminant and donkey raw milk samples and traditional dairy products in Iran.TheScientificWorldJournal · 2012Article
- Prevalence and characterization of non-O157 shiga toxin-producing Escherichia coli isolates from commercial ground beef in the United States.Applied and environmental microbiology · 2011Article
- Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China.BMC microbiology · 2010Article
- Molecular analysis of the Enterobacter sakazakii O-antigen gene locus.Applied and environmental microbiology · 2008Article
- Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Escherichia coli O45 O-antigen gene cluster of strain O45:H2 96-3285 was sequenced, and conventional (singleplex), multiplex, and real-time PCR assays were designed to amplify regions in the wzx (O-antigen flippase) and wzy (O-antigen polymerase) genes. In addition, PCR assays targeting the E. coli O55 wzx and wzy genes were designed based on previously published sequences. PCR assays targeting E. coli O45 showed 100% specificity for this serogroup, whereas by PCR assays specific for E. coli O55, 97/102 strains serotyped as E. coli O55 were positive for wzx and 98/102 for wzy. Multiplex PCR assays targeting the E. coli O45 and the E. coli O55 wzx and wzy genes were used to detect the organisms in fecal samples spiked at levels of 10(6) and 10(8) CFU/0.2 g feces. Thus, the PCR assays can be used to detect and identify E. coli serogroups O45 and O55.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.