Evidence map›Paper›PMID 16085892›Full record

ArticleApplied and environmental microbiology2005

Insertional inactivation of determinants for Mg2+ and Co2+ transport as a tool for screening recombinant Lactococcus species clones.

Susan Mills, Aidan Coffey, Colin Hill, Gerald F Fitzgerald, Olivia McAuliffe, R Paul Ross

Open access · greenAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Susan MillsTeagasc, Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Ireland.
Aidan Coffey
Colin Hill
Gerald F Fitzgerald
Olivia McAuliffe
R Paul Ross
University College Cork · IETeagasc - The Irish Agriculture and Food Development Authority · IEMunster Technological University · IE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones.

Indexed as

Mutagenesis, InsertionalBacterial ProteinsBiological Transport, ActiveCation Transport ProteinsCloning, MolecularCobaltGene Expression Regulation, BacterialLactococcusMagnesiumOpen Reading FramesPlasmidsRecombinant ProteinsBacterial ProteinsCation Transport ProteinsCobaltCorA protein, SalmonellaMagnesiumRecombinant Proteins

Identifiers

PMID16085892
PMCPMC1183277
OpenAlexW2161377568

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.