ArticleApplied and environmental microbiology2005
Aphid-symbiotic bacteria cultured in insect cell lines.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 82 citations in OpenAlex.
- Article
- Neuroprotective role ofFrontiers in nutrition · 2026Article
- Medical Potential of Insect Symbionts.Insects · 2025Review
- Characterization of heat, salt, acid, alkaline, and antibiotic stress response in soil isolate Bacillus subtilis strain PSK.A2.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Microbial production and functional assessment ofFrontiers in microbiology · 2025Article
- Quantification using a HPLC-FLD-based Detector and Molecular Identification of Ochratoxin-A ProducingRecent advances in food, nutrition & agriculture · 2025Article
- Intracellular defensive symbiont is culturable and capable of transovarial, vertical transmission.mBio · 2024Article
- Optimization of Siderophore Production in Three Marine Bacterial Isolates along with Their Heavy-Metal Chelation and Seed Germination Potential Determination.Microorganisms · 2023Article
- Bioprospecting Microalgae from Sewage Water: Assessment of Biochemicals for Biomass Utilization.Molecular biotechnology · 2023Article
- Diversity and dynamics of endosymbionts in a single population of sweet potato weevil, Cylas formicarius (Coleoptera: Brentidae): a preliminary study.Journal of insect science (Online) · 2023Article
- Efficient production and characterization of melanin fromFrontiers in microbiology · 2023Article
- Production and characterization of polyhydroxyalkanoates from industrial waste using soil bacterial isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Characterization and Cytotoxicity ofFrontiers in bioengineering and biotechnology · 2021Article
- Growing Ungrowable Bacteria: Overview and Perspectives on Insect Symbiont Culturability.Microbiology and molecular biology reviews : MMBR · 2020Review
- Antibacterial and cytotoxic metabolites of termite-associated Streptomyces sp. BYF63.The Journal of antibiotics · 2020Article
- Impact of Facultative Bacteria on the Metabolic Function of an Obligate Insect-Bacterial Symbiosis.mBio · 2020Article
- Evolution from Free-Living Bacteria to Endosymbionts of Insects: Genomic Changes and the Importance of the Chaperonin GroEL.Results and problems in cell differentiation · 2020Article
- New Insights into the Nature of Symbiotic Associations in Aphids: Infection Process, Biological Effects, and Transmission Mode of CultivableApplied and environmental microbiology · 2019Article
- Culture of an aphid heritable symbiont demonstrates its direct role in defence against parasitoids.Proceedings. Biological sciences · 2017Article
- Ecological impact of a secondary bacterial symbiont on the clones of Sitobion avenae (Fabricius) (Hemiptera: Aphididae).Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The cells and tissues of many aphids contain bacteria known as "secondary symbionts," which under specific environmental circumstances may be beneficial to the host insect. Such symbiotic bacteria are traditionally described as intractable to cultivation in vitro. Here we show that two types of aphid secondary symbionts, known informally as T type and U type, can be cultured and maintained in three insect cell lines. The identities of the cultured bacteria were confirmed by PCR with sequencing of 16S rRNA gene fragments and fluorescence in situ hybridization. In cell lines infected with bacteria derived from aphids harboring both T type and U type, the U type persisted, while the T type was lost. We suggest that the two bacteria persist in aphids because competition between them is limited by differences in tropism for insect tissues or cell types. The culture of these bacteria in insect cell lines provides a new and unique research opportunity, offering a source of unibacterial material for genomic studies and a model system to investigate the interactions between animal cells and bacteria. We propose the provisional taxon names "Candidatus Consessoris aphidicola" for T type and "Candidatus Adiaceo aphidicola" for U type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.