ArticleApplied and environmental microbiology2005
Shewanella oneidensis MR-1 uses overlapping pathways for iron reduction at a distance and by direct contact under conditions relevant for Biofilms.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 325 citations in OpenAlex.
- Expression and Engineering of Conductive Cytochrome Nanowires.bioRxiv : the preprint server for biology · 2026Article
- Magnetometry for Agriculture and Animal Systems: From Classical Sensors to Quantum-Enabled Biosensing.Biosensors · 2026Review
- Oxic microbial ferrihydrite reduction rates of Shewanella oneidensis and the potential for Fe mobilization in oxic sediments.Scientific reports · 2025Article
- Expanded Diversity of Microbial Groups Capable of Anaerobic Pyrite Reduction and Assimilation of Dissolution Products.Environmental microbiology · 2025Article
- Efficient genetic manipulation ofApplied and environmental microbiology · 2025Article
- Robust measurement of microbial reduction of graphene oxide nanoparticles using image analysis.Applied and environmental microbiology · 2025Article
- Shewanella oneidensis: Biotechnological Application of Metal-Reducing Bacteria.Advances in biochemical engineering/biotechnology · 2025Review
- Reduction of selenite and tellurite by a highly metal-tolerant marine bacterium.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Enhancing iron biogeochemical cycling forFrontiers in microbiology · 2024Article
- Moving towards the enhancement of extracellular electron transfer in electrogens.World journal of microbiology & biotechnology · 2023Review
- Agent-based modelling of iron cycling bacteria provides a framework for testing alternative environmental conditions and modes of action.Royal Society open science · 2022Article
- Genome-Scale Mutational Analysis of Cathode-OxidizingFrontiers in microbiology · 2022Article
- Biocorrosion on Nanofilms Induces Rapid Bacterial Motions via Iron Dissolution.ACS central science · 2021Article
- Identification of a pathway for electron uptake in Shewanella oneidensis.Communications biology · 2021Article
- Engineering S. oneidensis for Performance Improvement of Microbial Fuel Cell-a Mini Review.Applied biochemistry and biotechnology · 2021Review
- Electronic control of redox reactions inside Escherichia coli using a genetic module.PloS one · 2021Article
- Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms.Microorganisms · 2020Review
- A Hybrid Extracellular Electron Transfer Pathway Enhances the Survival of Vibrio natriegens.Applied and environmental microbiology · 2020Article
- Cellulose Aerogel Derived Hierarchical Porous Carbon for Enhancing Flavin-Based Interfacial Electron Transfer in Microbial Fuel Cells.Polymers · 2020Article
- Thermophiles; or, the Modern Prometheus: The Importance of Extreme Microorganisms for Understanding and Applying Extracellular Electron Transfer.Frontiers in microbiology · 2019Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a new method to measure iron reduction at a distance based on depositing Fe(III) (hydr)oxide within nanoporous glass beads. In this "Fe-bead" system, Shewanella oneidensis reduces at least 86.5% of the iron in the absence of direct contact. Biofilm formation accompanies Fe-bead reduction and is observable both macro- and microscopically. Fe-bead reduction is catalyzed by live cells adapted to anaerobic conditions, and maximal reduction rates require sustained protein synthesis. The amount of reactive ferric iron in the Fe-bead system is available in excess such that the rate of Fe-bead reduction is directly proportional to cell density; i.e., it is diffusion limited. Addition of either lysates prepared from anaerobic cells or exogenous electron shuttles stimulates Fe-bead reduction by S. oneidensis, but iron chelators or additional Fe(II) do not. Neither dissolved Fe(III) nor electron shuttling activity was detected in culture supernatants, implying that the mediator is retained within the biofilm matrix. Strains with mutations in omcB or mtrB show about 50% of the wild-type levels of reduction, while a cymA mutant shows less than 20% of the wild-type levels of reduction and a menF mutant shows insignificant reduction. The Fe-bead reduction defect of the menF mutant can be restored by addition of menaquinone, but menaquinone itself cannot stimulate Fe-bead reduction. Because the menF gene encodes the first committed step of menaquinone biosynthesis, no intermediates of the menaquinone biosynthetic pathway are used as diffusible mediators by this organism to promote iron reduction at a distance. CymA and menaquinone are required for both direct and indirect mineral reduction, whereas MtrB and OmcB contribute to but are not absolutely required for iron reduction at a distance.
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