ArticleApplied and environmental microbiology2005
Secretion of human serum albumin by Kluyveromyces lactis overexpressing KlPDI1 and KlERO1.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- A Lactose-BasedChem & bio engineering · 2025Article
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- Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints.Nature communications · 2022Article
- Secretory helpers for enhanced production of heterologous proteins inBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.Microbial cell factories · 2021Review
- Article
- Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin inBiomolecules · 2019Article
- Article
- Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.Microbial biotechnology · 2016Article
- Construction of a Kluyveromyces lactis ku80⁻ host strain for recombinant protein production: extracellular secretion of pectin lyase and a streptavidin-pectin lyase chimera.Molecular biotechnology · 2014Article
- Efficient secretion of lipase r27RCL in Pichia pastoris by enhancing the disulfide bond formation pathway in the endoplasmic reticulum.Journal of industrial microbiology & biotechnology · 2013Article
- Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production.Biotechnology for biofuels · 2013Article
- Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris.Microbial cell factories · 2011Article
- How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis.Biotechnology for biofuels · 2011Article
- SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion.Applied and environmental microbiology · 2008Article
- Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.Microbial cell factories · 2008Article
- Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts.Applied and environmental microbiology · 2007Article
- Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains.Applied and environmental microbiology · 2007Article
- A novel high-throughput screen reveals yeast genes that increase secretion of heterologous proteins.Applied and environmental microbiology · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The control of protein conformation during translocation through the endoplasmic reticulum is often a bottleneck for heterologous protein production. The core pathway of the oxidative folding machinery includes two conserved proteins: Pdi1p and Ero1p. We increased the dosage of the genes encoding these proteins in the yeast Kluyveromyces lactis and evaluated the secretion of heterologous proteins. KlERO1, an orthologue of Saccharomyces cerevisiae ERO1, was cloned by functional complementation of the ts phenotype of an Scero1 mutant. The expression of KlERO1 was induced by treatment of the cells with dithiothreitol and by overexpression of human serum albumin (HSA), a disulfide bond-rich protein. Duplication of either PDI1 or ERO1 led to a similar increase in HSA yield. Duplication of both genes accelerated the secretion of HSA and improved cell growth rate and yield. Increasing the dosage of KlERO1 did not affect the production of human interleukin 1beta, a protein that has no disulfide bridges. The results confirm that the ERO1 genes of S. cerevisiae and K. lactis are functionally similar even though portions of their coding sequence are quite different and the phenotypes of mutants overexpressing the genes differ. The marked effects of KlERO1 copy number on the expression of heterologous proteins with a high number of disulfide bridges suggests that control of KlERO1 and KlPDI1 is important for the production of high levels of heterologous proteins of this type.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.