ArticleApplied and environmental microbiology2005
Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- EngineeringiScience · 2026Article
- Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming.mBio · 2022Article
- A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility inPathogens (Basel, Switzerland) · 2022Article
- Manganese and cobalt recovery by surface display of metal binding peptide on various loops of OmpC in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Escherichia coli surface display for the selection of nanobodies.Microbial biotechnology · 2017Review
- Variability in bacterial flagella re-growth patterns after breakage.Scientific reports · 2017Article
- Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli.Microbial cell factories · 2010Article
- Developments in high-yield system expressed vaccines and immunotherapy.Recent patents on biotechnology · 2010Review
- The flagella of an atypical enteropathogenic Escherichia coli strain are required for efficient interaction with and stimulation of interleukin-8 production by enterocytes in vitro.Infection and immunity · 2009Article
- Development of a cell surface display system in a magnetotactic bacterium, "Magnetospirillum magneticum" AMB-1.Applied and environmental microbiology · 2008Article
- Use of transposon promoter-probe vectors in the metabolic engineering of the obligate methanotroph Methylomonas sp. strain 16a for enhanced C40 carotenoid synthesis.Applied and environmental microbiology · 2007Article
- Transcriptional and translational control of the Salmonella fliC gene.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial flagellum is composed of more than 20 different proteins. The filament, which constitutes the major extracellular part of the flagellum, is built up of approximately 20,000 FliC molecules that assemble at the growing distal end of the filament. A capping structure composed of five FliD molecules located at the tip of the filament promotes polymerization of FliC. Lack of FliD leads to release of the subunits into the growth medium. We show here that FliD can be successfully used in bacterial surface display. We tested various insertion sites in the capping protein, and the optimal region for display was at the variable region in FliD. Deletion and/or insertion at other sites resulted in decreased formation of flagella. We further developed the technique into a multihybrid display system in which three foreign peptides are simultaneously expressed within the same flagellum, i.e., D repeats of FnBPA from Staphylococcus aureus at the tip and fragments of YadA from Yersinia enterocolitica as well as SlpA from Lactobacillus crispatus along the filament. This technology can have biotechnological applications, e.g., in simultaneous delivery of several effector molecules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.