ArticleBiophysical journal2005
Variable incidence angle fluorescence interference contrast microscopy for z-imaging single objects.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Leucine zipper-based SAIM imaging identifies therapeutic agents to disrupt the cancer cell glycocalyx for enhanced immunotherapy.bioRxiv : the preprint server for biology · 2024Article
- Alternative molecular mechanisms for force transmission at adherens junctions via β-catenin-vinculin interaction.Nature communications · 2024Article
- Immunoengineering can overcome the glycocalyx armour of cancer cells.Nature materials · 2024Article
- MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells.Communications biology · 2023Article
- Glycocalyx Curving the Membrane: Forces Emerging from the Cell Exterior.Annual review of cell and developmental biology · 2021Review
- Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy.Molecular biology of the cell · 2018Article
- Dimensions and Interactions of Large T-Cell Surface Proteins.Frontiers in immunology · 2018Review
- Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking.Nature communications · 2017Article
- Revealing the Cell-Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy.ACS nano · 2017Article
- Nanoscale architecture of cadherin-based cell adhesions.Nature cell biology · 2017Article
- An acquisition and analysis pipeline for scanning angle interference microscopy.Nature methods · 2016Article
- Visualizing mechanical modulation of nanoscale organization of cell-matrix adhesions.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Talin determines the nanoscale architecture of focal adhesions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths.Faraday discussions · 2013Article
- Scanning angle interference microscopy reveals cell dynamics at the nanoscale.Nature methods · 2012Article
- DNA-tethered membranes formed by giant vesicle rupture.Journal of structural biology · 2009Article
- Quantification of nano-scale intermembrane contact areas by using fluorescence resonance energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A membrane interferometer.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Characterization of physical properties of supported phospholipid membranes using imaging ellipsometry at optical wavelengths.Biophysical journal · 2007Article
- Use of TIRF to Monitor T-Lymphocyte Membrane Dynamics with Submicrometer and Subsecond Resolution.Cellular and molecular bioengineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Surface-generated structured illumination microscopies interrogate the position of fluorescently labeled objects near surfaces with nanometer resolution along the z axis. However, these techniques are either experimentally cumbersome or applicable to a limited set of experimental systems. We present a new type of surface-generated structured illumination fluorescence microscopy, variable incidence angle fluorescence interference contrast microscopy (VIA-FLIC), in which the fluorescent sample is assembled above a reflective Si surface and the incidence angle of excitation light is varied by placing annular photomasks with different radii in the aperture diaphragm plane of the microscope. The variation in incidence angle alters the interference pattern of excitation light, and hence the intensity of detected fluorescence. Quantitative VIA-FLIC is tested by using a set of fluorophore-containing supported membranes separated from the Si surface by SiO2 layers of variable thicknesses. The resulting fluorescence intensity versus incidence angle curves depends on the separation from the Si surface and when fit with an appropriate model yield precise SiO2 thicknesses that are accurate with respect to the known SiO2 thicknesses. Since only a simple modification to a standard epifluorescence microscope is required, VIA-FLIC offers a versatile method to produce z-reconstructions with high resolution for a wide range of biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.