ArticleJournal of immunological methods2005
Laser capture microdissection and single-cell RT-PCR without RNA purification.
Article in Journal of immunological methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Review
- Dielectrophoretic capture and electrochemical enzyme-linked immunosorbent assay of single melanoma cells at an array of interlocked spiral bipolar electrodes.ChemElectroChem · 2024Article
- Differential Regulation of Neurotrophic Factors During Pathogenic Tau-Aggregation in a Tau Transgenic Mouse Model for Alzheimer's Disease: A Protocol for Double-Labeling mRNA by In Situ Hybridization and Protein Epitopes by Immunohistochemistry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis.Frontiers in immunology · 2022Review
- Single-cell RNA sequencing in breast cancer: Understanding tumor heterogeneity and paving roads to individualized therapy.Cancer communications (London, England) · 2020Review
- Cell differentiation in cardiac myxomas: confocal microscopy and gene expression analysis after laser capture microdissection.Heart and vessels · 2018Article
- Global and targeted approaches to single-cell transcriptome characterization.Briefings in functional genomics · 2018Article
- A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.Journal of immunological methods · 2017Article
- Studying hematopoiesis using single-cell technologies.Journal of hematology & oncology · 2017Review
- The niche in single-cell technologies.Immunology and cell biology · 2016Review
- Technologies for Single-Cell Isolation.International journal of molecular sciences · 2015Review
- Alternative splicing of human insulin receptor gene (INSR) in type I and type II skeletal muscle fibers of patients with myotonic dystrophy type 1 and type 2.Molecular and cellular biochemistry · 2013Article
- The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum.Epidemiology and infection · 2013Article
- Rapid expression profiling of brain microvascular endothelial cells by immuno-laser capture microdissection coupled to TaqMan(®) low density array.Journal of neuroscience methods · 2012Article
- Laser capture microdissection of Drosophila peripheral neurons.Journal of visualized experiments : JoVE · 2010Article
- Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.BMC molecular biology · 2008Article
- Elevated alpha-synuclein mRNA levels in individual UV-laser-microdissected dopaminergic substantia nigra neurons in idiopathic Parkinson's disease.Nucleic acids research · 2008Article
- Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues.BMC biotechnology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Chronic infectious diseases of the central nervous system (CNS) are characterized by intrathecal synthesis of increased amounts of immunoglobulin G (IgG) directed against the agent that causes disease. In other inflammatory CNS diseases such as multiple sclerosis and CNS sarcoid, the targets of the humoral immune response are uncertain. To identify the IgGs expressed by individual CD38(+) plasma cells seen in human brain sections, we merged the techniques of laser capture microdissection (LCM) and single-cell RT-PCR. Frozen brain sections from a patient who died of subacute sclerosing panencephalitis (SSPE), were rapidly immunostained and examined by LCM to dissect individual CD38(+) cells. After cell lysis, we developed two techniques for reverse-transcription (RT) of unpurified total RNA in the cell lysates. The first method performed repeated and rapid freeze-thawing, followed by centrifugation of the cell lysate into tubes for subsequent RT. The second, more successful method performed RT in situ on detergent-solubilized cells directly on the cap surface; subsequent nested PCR identified heavy and light chain sequences expressed by two-thirds of individually isolated plasma cells. These techniques will streamline the identification of gene expression products in single cells from complex tissues and have the potential to identify IgGs expressed in the CNS of inflammatory diseases of unknown etiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.