ArticleJournal of clinical microbiology2005
New microsatellite multiplex PCR for Candida albicans strain typing reveals microevolutionary changes.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 133 citations in OpenAlex.
- Article
- The Gastrointestinal Tract Is Pinpointed as a Reservoir ofJournal of fungi (Basel, Switzerland) · 2023Article
- Biased Genotype Distributions of Candida albicans Strains Associated with 649 Clinical Vulvovaginal Candidiasis in China.Mycopathologia · 2022Article
- Genotyping ofCurrent medical mycology · 2022Article
- Article
- Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- OralJournal of oral microbiology · 2020Article
- Site-Specific Profiling of the Dental Mycobiome Reveals Strong Taxonomic Shifts during Progression of Early-Childhood Caries.Applied and environmental microbiology · 2020Article
- Genotyping Reveals High Clonal Diversity and Widespread Genotypes ofFrontiers in cellular and infection microbiology · 2020Article
- Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations.Yeast (Chichester, England) · 2019Article
- Genetic diversity and antifungal susceptibility of Candida albicans isolated from Iranian patients.Medical mycology · 2019Article
- Longitudinal Survey of Fungi in the Human Gut: ITS Profiling, Phenotyping, and Colonization.Frontiers in microbiology · 2019Article
- Cariogenicity of Candida albicans of distinct genotypes among 3-5-year-old Uygur children in Kashgar, China- a case-control study.BMC oral health · 2018Article
- Polymorphism of Polymeric Amino Acid Regions in Fungal Proteins and Correlation with Altered Echinocandin and Azole Susceptibility.Antimicrobial agents and chemotherapy · 2018Article
- Reduction in Percentage of Clusters of Candida albicans and Candida parapsilosis Causing Candidemia in a General Hospital in Madrid, Spain.Journal of clinical microbiology · 2018Article
- Multi-Locus Next-Generation Sequence Typing of DNA Extracted From Pooled Colonies Detects Multiple UnrelatedFrontiers in microbiology · 2018Article
- Article
- The intraspecies diversity of C. albicans triggers qualitatively and temporally distinct host responses that determine the balance between commensalism and pathogenicity.Mucosal immunology · 2017Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Molecular Fingerprinting Studies Do Not Support Intrahospital Transmission ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five new microsatellite loci were described and characterized for use as molecular markers for the identification and genetic differentiation of Candida albicans strains. Following the typing of 72 unrelated clinical isolates, the analysis revealed that they were all polymorphic, presenting from 5 to 30 alleles and 8 to 46 different genotypes. The discriminatory power obtained by combining the information generated by three microsatellites used in a multiplex PCR amplification strategy was 0.99, the highest ever reported. The multiplex PCR was later used to test a total of 114 C. albicans strains, including multiple isolates from the same patient collected from different body locations and along episodes of vulvovaginal infections. Three different scenarios for strain relatedness were identified: (i) different isolates that were revealed to be the same strain, (ii) isolates that were the same strain but that apparently underwent a process of microevolution, and (iii) isolates that corresponded to different strains. Analysis of the microevolutionary changes between isolates from recurrent infections indicated that the genotype alterations observed could be the result of events that lead to the loss of heterozygosity (LOH). In one case of recurrent infection, LOH was observed at the CAI locus, and this could have been related to exposure to fluconazole, since such strains were exposed to this antifungal during treatment. The analysis of microsatellites by a multiplex PCR strategy was found to be a highly efficient tool for the rapid and accurate differentiation of C. albicans strains and adequate for the identification of fine microevolutionary events that could be related to strain microevolution in response to environmental stress conditions.
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