ArticleJournal of clinical microbiology2005
DNA probe array for the simultaneous identification of herpesviruses, enteroviruses, and flaviviruses.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Progress in etiological diagnosis of viral meningitis.Frontiers in neurology · 2023Review
- Flavivirus Persistence in Wildlife Populations.Viruses · 2021Review
- Molecular and Immunological Diagnostic Techniques of Medical Viruses.International journal of microbiology · 2020Review
- A Novel Pan-BioMed research international · 2017Article
- DNA Microarray Platform for Detection and Surveillance of Viruses Transmitted by Small Mammals and Arthropods.PLoS neglected tropical diseases · 2016Article
- Article
- An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.PLoS neglected tropical diseases · 2014Article
- Evaluation of a field-portable DNA microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals.The American journal of tropical medicine and hygiene · 2013Article
- Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.PLoS neglected tropical diseases · 2013Article
- Emerging view of the human virome.Translational research : the journal of laboratory and clinical medicine · 2012Review
- Improvement of the specificity of a pan-viral microarray by using genus-specific oligonucleotides and reduction of interference by host genomes.Journal of medical virology · 2011Article
- Gene expression analysis in the thalamus and cerebrum of horses experimentally infected with West Nile virus.PloS one · 2011Article
- Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm.BMC genomics · 2010Article
- Identifying Fishes through DNA Barcodes and Microarrays.PloS one · 2010Article
- VIPR: A probabilistic algorithm for analysis of microbial detection microarrays.BMC bioinformatics · 2010Article
- Development of a consensus microarray method for identification of some highly pathogenic viruses.Journal of medical virology · 2009Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Typing of enteroviruses by use of microwell oligonucleotide arrays.Journal of clinical microbiology · 2009Article
- Identification of upper respiratory tract pathogens using electrochemical detection on an oligonucleotide microarray.PloS one · 2007Article
- Chapter 13 Recent Advances and Future Needs in Environmental Virology.Perspectives in medical virology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral infections of the central nervous system (CNS) are caused by a variety of viruses, namely, herpesviruses, enteroviruses, and flaviviruses. The similar clinical signs provoked by these viruses make the diagnosis difficult. We report on the simultaneous detection of these major CNS pathogens using amplification by PCR and detection of amplified products using DNA microarray technology. Consensus primers were used for the amplification of all members of each genus. Sequences specific for the identification of each virus species were selected from the sequence alignments of each target gene and were synthesized on a high-density microarray. The amplified products were pooled, labeled, and cleaved, followed by hybridization on a single array. This method was successfully used to identify herpesviruses, namely, herpes simplex virus type 1 (HSV-1), HSV-2, and cytomegalovirus; all serotypes of human enteroviruses; and five flaviviruses (West Nile virus, dengue viruses, and Langat virus). This approach, which used highly conserved consensus primers for amplification and specific sequences for identification, would be extremely useful for the detection of variants and would probably help solve some unexplained cases of encephalitis. The analytical sensitivity of the method was shown to be 500 genome equivalents ml(-1) for HSV-1, 0.3 50% tissue culture infectious doses (TCID50s) ml(-1) for the enterovirus coxsackievirus A9, and 200 TCID50s ml(-1) for West Nile virus. The clinical sensitivity of this method must now be evaluated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.