ArticleJournal of clinical microbiology2005
Development of a new oligonucleotide array to identify staphylococcal strains at species level.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Biopolymer production by halotolerant bacteria isolated from Caatinga biome.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Universal Probe Library based real-time PCR for rapid detection of bacterial pathogens from positive blood culture bottles.World journal of microbiology & biotechnology · 2014Article
- Identification of a variety of Staphylococcus species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2010Article
- fbl gene as a species-specific target for Staphylococcus lugdunensis identification.Journal of clinical laboratory analysis · 2010Article
- A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin.BMC microbiology · 2009Article
- A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae.FEMS yeast research · 2009Article
- Identification of coagulase-negative Staphylococci by using the BD phoenix system in the low-inoculum mode.Journal of clinical microbiology · 2008Article
- Exposure to bioaerosols in poultry houses at different stages of fattening; use of real-time PCR for airborne bacterial quantification.The Annals of occupational hygiene · 2008Article
- Evaluation of the Vitek 2 system with a variety of Staphylococcus species.Journal of clinical microbiology · 2008Article
- Use of a DNA microarray for simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates.Journal of clinical microbiology · 2007Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genus Staphylococcus is made up of 36 validated species which contain strains that are pathogenic, saprophytic, or used as starter cultures for the food industry. An oligonucleotide array targeting the manganese-dependent superoxide dismutase (sodA) gene was developed to overcome the drawbacks of the conventional methods of identification. Divergences of the sodA gene were used to design oligonucleotide probes, and we showed that each of the 36 species had a characteristic pattern of hybridization. To evaluate the array, we analyzed 38 clinical and 38 food or food plant Staphylococcus isolates identified by the phenotype-based system VITEK 2 (bioMérieux). This commercial kit failed to identify 8 (21%) of the clinical isolates and 32 (84%) of the food and food plant isolates. In contrast, the oligonucleotide array we designed provided an accurate and rapid method for the identification of staphylococcal strains, isolated from clinical, environmental, or food samples, at species level.
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