ArticleJournal of clinical microbiology2005
Sensitivity of PCR targeting the IS2404 insertion sequence of Mycobacterium ulcerans in an Assay using punch biopsy specimens for diagnosis of Buruli ulcer.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Efficacy of the combination rifampin-streptomycin in preventing growth of Mycobacterium ulcerans in early lesions of Buruli ulcer in humans.Antimicrobial agents and chemotherapy · 2005Trial
- A rapid and simple MALDI-TOF MS lipid profiling method for differentiatingJournal of clinical microbiology · 2025Article
- Buruli ulcer: Current landscape, challenges, and future directions.Journal of clinical tuberculosis and other mycobacterial diseases · 2025Article
- Review
- Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.Journal of fish diseases · 2023Article
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Evaluation of the fluorescent-thin layer chromatography (f-TLC) for the diagnosis of Buruli ulcer disease in Ghana.PloS one · 2022Article
- Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics.iScience · 2021Article
- Understanding the transmission of Mycobacterium ulcerans: A step towards controlling Buruli ulcer.PLoS neglected tropical diseases · 2021Review
- Global Epidemiology of Buruli Ulcer, 2010-2017, and Analysis of 2014 WHO Programmatic Targets.Emerging infectious diseases · 2019Article
- Rapid Extraction Method ofDiagnostics (Basel, Switzerland) · 2019Article
- Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay.PLoS neglected tropical diseases · 2019Article
- Diagnostic Accuracy of Clinical and Microbiological Signs in Patients With Skin Lesions Resembling Buruli Ulcer in an Endemic Region.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- The chemistry and biology of mycolactones.Beilstein journal of organic chemistry · 2017Review
- Spatial Distribution of Mycobacterium ulcerans in Buruli Ulcer Lesions: Implications for Laboratory Diagnosis.PLoS neglected tropical diseases · 2016Article
- Article
- Detection of Mycobacterium ulcerans by real-time PCR with improved primers.Tropical medicine and health · 2016Article
- Clinical and Laboratory Diagnosis of Buruli Ulcer Disease: A Systematic Review.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2016Review
- Metabolomic profiles delineate mycolactone signature in Buruli ulcer disease.Scientific reports · 2015Article
- Simple, Rapid Mycobacterium ulcerans Disease Diagnosis from Clinical Samples by Fluorescence of Mycolactone on Thin Layer Chromatography.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Punch biopsy specimens from Mycobacterium ulcerans disease lesions were used to compare the sensitivities and specificities of direct smear, culture, PCR, and histopathology in making a diagnosis of M. ulcerans disease in a field setting. PCR for the insertion element IS2404 was modified to include uracil-N-glycosylase and deoxyuridine triphosphate instead of deoxythymidine triphosphate to reduce the risk of cross contamination. The "gold standard" for confirmation of clinically diagnosed Buruli ulcer was a definite histological diagnosis, a positive culture for M. ulcerans, or a smear positive for acid-fast bacilli (AFB), together with a possible histological diagnosis. For 70 clinically diagnosed cases of M. ulcerans disease, the modified PCR was 98% sensitive and gave a rapid result. The sensitivities of microscopy, culture, and histology were 42%, 49%, and 82%, respectively. The use of a 4-mm punch biopsy specimen was preferred to a 6-mm punch biopsy specimen since the wound was less likely to bleed and to need stitching. Given adequate technical expertise and the use of controls, the PCR was viable in a teaching hospital setting in Ghana; and in routine practice, we would recommend the use of Ziehl-Neelsen staining of biopsy specimens to detect AFB, followed by PCR, in AFB-negative cases only, in order to minimize costs. Histology and culture remain important as quality control tests, particularly in studies of treatment efficacy.
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