ArticleGenetics2005
Multiple bromodomain genes are involved in restricting the spread of heterochromatic silencing at the Saccharomyces cerevisiae HMR-tRNA boundary.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 62 citations in OpenAlex.
- ATAD2 as a Cancer Target: Insights into Its Structure, Functions, Mechanisms, and Drug Development.Cancers · 2025Review
- Structure of the human ATAD2 AAA+ histone chaperone reveals mechanism of regulation and inter-subunit communication.Communications biology · 2023Article
- Article
- Tumor-Promoting ATAD2 and Its Preclinical Challenges.Biomolecules · 2022Review
- The ATAD2/ANCCA homolog Yta7 cooperates with Scm3Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Proteomic profiling of yeast heterochromatin connects direct physical and genetic interactions.Current genetics · 2019Article
- Regulation of DNA replication-coupled histone gene expression.Oncotarget · 2017Review
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing.Nature communications · 2016Article
- Interplay between histone H3 lysine 56 deacetylation and chromatin modifiers in response to DNA damage.Genetics · 2015Article
- Lessons from yeast on emerging roles of the ATAD2 protein family in gene regulation and genome organization.Molecules and cells · 2014Review
- Chromosome boundary elements and regulation of heterochromatin spreading.Cellular and molecular life sciences : CMLS · 2014Review
- Chromatin remodelling complex RSC promotes base excision repair in chromatin of Saccharomyces cerevisiae.DNA repair · 2014Article
- Regulation of histone gene transcription in yeast.Cellular and molecular life sciences : CMLS · 2014Review
- Intergenic transcriptional interference is blocked by RNA polymerase III transcription factor TFIIIB in Saccharomyces cerevisiae.Genetics · 2014Article
- Direct interplay among histones, histone chaperones, and a chromatin boundary protein in the control of histone gene expression.Molecular and cellular biology · 2012Article
- TFIIIC localizes budding yeast ETC sites to the nuclear periphery.Molecular biology of the cell · 2012Article
- Human tRNA genes function as chromatin insulators.The EMBO journal · 2012Article
- Direct regulation of nucleosome density by the conserved AAA-ATPase Yta7.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Dot1 binding induces chromatin rearrangements by histone methylation-dependent and -independent mechanisms.Epigenetics & chromatin · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transfer RNA gene downstream from the HMR locus in S. cerevisiae functions as part of a boundary (barrier) element that restricts the spread of heterochromatic gene silencing into the downstream region of chromosome III. A genetic screen for identifying additional genes that, when mutated, allow inappropriate spreading of silencing from HMR through the tRNA gene was performed. YTA7, a gene containing bromodomain and ATPase homologies, was identified multiple times. Previously, others had shown that the bromodomain protein Bdf1p functions to restrict silencing at yeast euchromatin-heterochromatin boundaries; therefore we deleted nonessential bromodomain-containing genes to test their effects on heterochromatin spreading. Deletion of RSC2, coding for a component of the RSC chromatin-remodeling complex, resulted in a significant spread of silencing at HMR. Since the bromodomain of YTA7 lacks a key tyrosine residue shown to be important for acetyllysine binding in other bromodomains, we confirmed that a GST-Yta7p bromodomain fusion was capable of binding to histones in vitro. Epistasis analysis suggests that YTA7 and the HMR-tRNA function independently to restrict the spread of silencing, while RSC2 may function through the tRNA element. Our results suggest that multiple bromodomain proteins are involved in restricting the propagation of heterochromatin at HMR.
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